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首页> 外文期刊>Phytochemical Analysis >One-step preparative separation of two polyhydroxystilbenes from Rheum likiangense Sam. by high-speed counter-current chromatography.
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One-step preparative separation of two polyhydroxystilbenes from Rheum likiangense Sam. by high-speed counter-current chromatography.

机译：从大黄中分离出两种多羟基对苯二酚的一步制备。通过高速逆流色谱法。

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摘要

Introduction - The official rhubarb species have frequently been investigated for their hydroxyanthraquinone components and associated pharmacological properties. However, other unofficial rhubarb species were rarely studied until polyhydroxystilbenes (PHS), which commonly occur in the unofficial rhubarb, revealed a range of potential bioactivities. Hence, there has been increasing interest in the efficient preparation of high-purity PHS for pharmacological and clinical trials. Objective - To develop a suitable method for large-scale preparative separation of PHS from the rhizome of Rheum likiangense Sam. by high-speed counter-current chromatography (HSCCC). Results - Two PHS compounds were isolated successfully within 440 min using a solvent system consisting of methanol:n-butanol:chloroform:water (2:0.5:3:3, v/v/v/v). Eighty-four milligrams of desoxyrhaponticin with 98.2% purity and 148 mg of rhaponticin with 95.3% purity were respectively yielded from 1.5 g of crude extract in the single, one-step operation. Conclusion - An optimised HSCCC method has been established for large-scale preparative separation of PHS compounds from Rheum likiangense Sam.
机译：简介-官方研究了大黄种类中的羟基蒽醌成分及其相关药理特性。但是，很少研究其他非官方的大黄物种，直到通常在非官方的大黄中出现的多羟基对苯二酚（PHS）揭示了一系列潜在的生物活性。因此，对于用于药理和临床试验的高纯度PHS的有效制备越来越引起人们的兴趣。目的-建立一种从大黄大黄的根茎中大规模分离制备PHS的合适方法。通过高速逆流色谱（HSCCC）。结果-使用由甲醇：正丁醇：氯仿：水（2：0.5：3：3，v / v / v / v）组成的溶剂系统，在440分钟内成功分离出两种PHS化合物。从1.5 g粗提物中进行的一步一步操作分别得到84毫克纯度为98.2％的脱氧皂苷和148毫克纯度为95.3％的鼠李糖苷。结论-已经建立了一种优化的HSCCC方法，用于从大黄中大规模分离制备PHS化合物。

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