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首页> 外文期刊>Phytochemical Analysis >Ultra-performance liquid chromatography-high-definition mass spectrometry analysis of constituents in the root of Radix Stemonae and those absorbed in blood after oral administration of the extract of the crude drug.
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Ultra-performance liquid chromatography-high-definition mass spectrometry analysis of constituents in the root of Radix Stemonae and those absorbed in blood after oral administration of the extract of the crude drug.

机译：超高效液相色谱-高分辨质谱法分析口服药材提取物后，何首乌的根成分和血液中吸收的成分。

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Introduction - Radix Stemonae, first recorded in the herbal 'Additional Records of Famous Physicians' (Mingyi Bielu), has been widely used as an oral anti-tussive agent in traditional Chinese medicine (TCM) for thousands of years in China. Current analysis methods are usually time-consuming and inaccurate. Objective - The aim of this study was to develop a more rapid and sensitive method to detect the active compounds in Radix Stemonae. Methodology - A rapid, sensitive and selective ultra-performance liquid chromatography-high-resolution mass spectrometric (UPLC-HDMS) method with automated data analysis software MetaboLynxTM was developed for analysis and identification of the bioactive components and their metabolites in rat plasma following oral administration of Radix Stemonae extract. Results - Twenty-nine compounds in the Radix Stemonae extract and 47 compounds in the rat plasma samples were detected in the MSE mode in a single injection within 21 min. Among the compounds detected, 23 were identified in vitro and 42 were identified in rat plasma, either by comparing the retention time and mass spectrometry data with those of reference compounds or by mass spectrometry analysis and retrieving the reference literature. Conclusion - It is concluded that the screening capability of MetaboLynxTM, together with the utilisation of MSE in structural elucidation, can facilitate a rapid and comprehensive detection and effective structural characterisation of Stemona alkaloids in Radix Stemonae extract, which could be applied to other Chinese medicine. The results provide helpful chemical information of Radix Stemonae extract for further pharmacology and active mechanism research.
机译：简介-丹参，最早记录在草药“著名医师附加记录”（Mingyi Bielu）中，在中国数千年来已广泛用作中药的口服镇咳药。当前的分析方法通常既费时又不准确。目的-这项研究的目的是开发一种更快速，更灵敏的方法来检测蒲黄中的活性化合物。方法-用自动数据分析软件MetaboLynx TM 开发了一种快速，灵敏和选择性的超高效液相色谱-高分辨率质谱（UPLC-HDMS）方法，用于分析和鉴定生物活性成分和口服丹参提取物后它们在大鼠血浆中的代谢产物。结果-在21分钟内以MS E 模式一次检测到了甜叶提取物中的29种化合物和大鼠血浆样品中的47种化合物。通过比较保留时间和质谱数据与参考化合物的保留时间和质谱数据，或者通过质谱分析并检索参考文献，在检测到的化合物中，在体外鉴定出23种化合物，在大鼠血浆中鉴定出42种化合物。结论-结论：MetaboLynx TM 的筛选能力以及MS E 在结构鉴定中的利用，可促进快速，全面的检测和有效的结构鉴定。何首乌提取物中的总碱生物碱，可用于其他中药。研究结果为板蓝根提取物的进一步化学研究和活性机理研究提供了有益的化学信息。

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