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首页> 外文期刊>Phytochemical Analysis >Improved procedure for the quality control of Hypericum perforatum L.
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Improved procedure for the quality control of Hypericum perforatum L.

机译：贯叶连翘的质量控制的改进程序。

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摘要

A new, fast and reliable procedure for the quantification of the major compounds of Hypericum perforatum L. has been developed. Four naphthodianthrones (protopseudohypericin, pseudohypericin, protohypericin, hypericin) and two phloroglucinols (hyperforin, adhyperforin) were assayed by HPLC using a short (17 min) linear gradient, with hypericin and hyperforin as external standards. Extraction of dried plant material with methanol in the dark at room temperature for 2 h led to a complete recovery of phloroglucinols but only a partial recovery of the naphthodianthrone derivatives. Treatment of plant material with water:ethanol (40:60, v/v) in a water bath shaker at 80 degreesC led to the total extraction of hypericins, but a 10% loss of total hyperforins was also observed. The two extraction methods, applied successively to the same sample, allowed the complete extraction of all compounds of interest. A 5 min exposure of the crude extract of H. perforatum to sunlight (1 E/m(2)) induced a 96% loss of hyperforins, whereas the dry plant material lost only 20% of hyperforins after 2 h exposure to sunlight (24 E/m(2)).
机译：已经开发了一种新的，快速而可靠的定量贯叶连翘的主要化合物的程序。 HPLC使用短时间（17分钟）线性梯度，以金丝桃素和金丝桃素为外标，通过高效液相色谱法测定了四种萘二酮（原拟高丝菌素，假高丝菌素，原高丝菌素，金丝桃素）和两个间苯三酚（金丝桃素，adhyperforin）。在室温下于黑暗中用甲醇萃取干燥的植物材料2小时，可以完全回收间苯三酚，但只能部分回收萘二蒽酮衍生物。在80℃的水浴振荡器中，用水：乙醇（40：60，v / v）处理植物材料可导致金丝桃素的总提取，但也观察到金丝桃素的总损失为10％。连续应用于相同样品的两种提取方法可以完全提取所有目标化合物。贯叶连翘粗提取物暴露于阳光下5分钟（1 E / m（2））导致hyperforins损失96％，而干燥的植物材料暴露于阳光2 h后仅损失20％的hyperforins（24 E / m（2））。

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