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A new approach to fabricate agarose microstructures

机译：一种制备琼脂糖微结构的新方法

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摘要

Agarose hydrogels find wide applications in different fields such as biological sciences, tissue engineering and food industry, and its use has been investigated in many fields ranging from electronics to crystallography. Usually, agarose structures are made by casting, and more recently some attempts have been made to build agarose structures by additive manufacturing. All of the fabrication methods are based on thermo-reversible gelling properties of agarose gel. A new method to fabricate agarose microstructures in a binary solvent composed of water and dimethyl sulfoxide is presented and modelled in this paper. This new method allows building agarose structures by an additive layer-by-layer approach using a modified inkjet printer. The fabrication method and printing device are described in detail. Furthermore, finite-element model simulations, which predict with high confidence the final line width of the printed structures, are discussed and analysed. Mechanical properties of printed gel structures are comparable with those obtained by gel casting, as demonstrated by tensile testing. The presented results demonstrate the feasibility of this approach to fabricate agarose structures with more complex shapes that can be done by casting.
机译：琼脂糖水凝胶在生物科学，组织工程和食品工业等不同领域有广泛的应用，其用途已从电子学到晶体学等许多领域得到了研究。通常，琼脂糖结构是通过铸造制成的，并且最近已经进行了一些尝试通过增材制造来构建琼脂糖结构的尝试。所有制造方法均基于琼脂糖凝胶的热可逆胶凝特性。本文提出了一种在水和二甲基亚砜组成的二元溶剂中制备琼脂糖微结构的新方法，并进行了建模。这种新方法允许使用改进的喷墨打印机通过逐层添加的方法构建琼脂糖结构。详细描述制造方法和打印装置。此外，讨论并分析了有限元模型仿真，该仿真以高可信度预测了印刷结构的最终线宽。如拉伸测试所示，印刷的凝胶结构的机械性能与通过凝胶浇铸获得的机械性能相当。提出的结果证明了这种方法制造具有更复杂形状的琼脂糖结构的可行性，该结构可以通过铸造来完成。

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