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首页> 外文期刊>Polymers for advanced technologies >Micropatterning of proteins on ion beam-induced poly(acrylic acid)-grafted polyethylene film
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Micropatterning of proteins on ion beam-induced poly(acrylic acid)-grafted polyethylene film

机译:离子束诱导的聚丙烯酸接枝聚乙烯薄膜上蛋白质的微图案化

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摘要

Micropatterns of proteins were created by using patterned ion beam irradiation onto a polyethylene film and graft polymerization of acrylic acid. Acrylic acid was selectively graft polymerized on the irradiated regions. The results of the grafting study revealed that the optimum fluence to achieve the maximum grafting degree was 1×10~(15) ions/cm~2. Biotin was covalently immobilized on the grafted regions of the polyethylene film. Protein patterning was achieved through specific binding of biotin and streptavidin. The resolved protein patterns with the maximum fluorescence intensity were achieved on the poly(acrylic acid) (PAA)-grafted polyethylene films prepared at the fluence of 1×10~(15) ions/cm~2. This method can be used for patterning of various biomolecules and for further biological applications.
机译:通过使用图案化的离子束辐照到聚乙烯薄膜上并接枝丙烯酸,可以创建蛋白质的微图案。丙烯酸被选择性地接枝聚合在被照射的区域上。接枝研究结果表明,达到最大接枝度的最佳通量为1×10〜(15)离子/ cm〜2。将生物素共价固定在聚乙烯膜的接枝区域上。通过生物素和链霉亲和素的特异性结合来实现蛋白质图案化。在1×10〜(15)离子/ cm〜2的注量制备的聚丙烯酸(PAA)接枝聚乙烯薄膜上,获得了具有最大荧光强度的分辨蛋白质模式。该方法可用于各种生物分子的图案化以及进一步的生物学应用。

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