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首页> 外文期刊>Polymers for advanced technologies >Poly(L-histidine) with several aminoethyl groups for a new pH-sensitive DNA carrier
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Poly(L-histidine) with several aminoethyl groups for a new pH-sensitive DNA carrier

机译：具有多个氨乙基的聚（L-组氨酸），用于新型pH敏感的DNA载体

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摘要

In this study, poly(L-histidine) with several aminoethyl groups, i.e. aminated poly(L-histidine), is reported to be able to make complexes with DNA and to transfect cells in vitro in the presence of serum. The present study was performed to determine whether the pH of the medium had an influence on the complex formation with DNA, on the cell membrane fusion activity and on the trans-fection efficiency. Agarose gel retardation assays proved that the polyion complex formation of the aminated poly(L-histidine) with DNA was affected by pH of the medium, owing to the basicity (protonation-deprotonation) of the imidazole groups with a pK{sub}a value around 6.0. Hemolysis assay showed that the resulting DNA complex enhanced membrane disruptive ability at endosomal pH. The aminated poly(L-histidine) gene carrier demonstrated significant transfection efficacy which was decreased by the inclusion of chloroquine as an endosomolytic agent. These results suggest that the aminated poly(L-histidine) promises to be a new pH-sensitive DNA carrier for endosomal escape.
机译：在该研究中，据报道具有多个氨基乙基的聚（L-组氨酸），即胺化的聚（L-组氨酸），能够与DNA形成复合物，并且能够在血清存在下体外转染细胞。进行本研究以确定培养基的pH是否对与DNA的复合物形成，细胞膜融合活性和转染效率有影响。琼脂糖凝胶阻滞试验证明，胺化的聚（L-组氨酸）与DNA的聚离子复合物形成受介质pH的影响，这是由于咪唑基团的碱性（质子化-去质子化）具有pK {sub} a值大约6.0。溶血分析表明，所得的DNA复合物在内体pH值下增强了膜破坏能力。胺化的聚（L-组氨酸）基因载体表现出显着的转染效率，但由于加入氯喹作为内溶菌剂而降低了转染效率。这些结果表明，胺化的聚（L-组氨酸）有望成为一种新的pH敏感的DNA载体，用于内体逃逸。

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