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首页> 外文期刊>Poultry Science >A single nucleotide polymorphism in the parathyroid hormone gene and effects on eggshell quality in chickens.
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A single nucleotide polymorphism in the parathyroid hormone gene and effects on eggshell quality in chickens.

机译：甲状旁腺激素基因中的单核苷酸多态性及其对鸡卵壳质量的影响。

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摘要

Parathyroid hormone (PTH), released by the parathyroid gland of animals, plays an important role in regulating the metabolism of calcium and phosphate. As a candidate gene for eggshell quality traits, the SNP was screened and its genetic effects on eggshell qualities and levels of serum calcium, phosphate, and PTH were analyzed in this study. Three hundred Houdan hens, an indigenous breed of chicken in France, were used for genotyping and data recording. Of the 3 sets of primers used to amplify the exons, exon 3 was polymorphic and 3 genotypes were identified. Sequencing revealed a nucleotide transition, A2205G (GenBank accession no. NC_006092), which was a synonymous mutation and caused a codon for lysine to change from AAA to AAG. Eggshell percentage and breaking strength for genotypes GG and AG were greater (P <0.05) than for AA, respectively; the total serum calcium for genotype GG was higher than for genotypes AA and AG by 11.8 and 10.1%, respectively; the serum phosphate for genotype GG was greater than genotype AA and AG by 16.7 and 12%, respectively. All genotypes shared the same calcium:phosphate ratio. For serum PTH, genotype GG was approximately 30% higher than genotype AA. Therefore, the SNP A2205G in PTH affected the eggshell percentage and breaking strength, and it may be associated with the variation of serum calcium and PTH level, indicating that the SNP in PTH has the potential for utilization in a MAS program for eggshell quality in chicken.
机译：动物的甲状旁腺释放的甲状旁腺激素（PTH）在调节钙和磷酸盐的代谢中起重要作用。作为蛋壳品质性状的候选基因，本研究筛选了SNP，并分析了其对蛋壳品质以及血清钙，磷酸盐和PTH水平的遗传影响。 300头法国母鸡Houdan母鸡用于基因分型和数据记录。在用于扩增外显子的3组引物中，外显子3是多态的，并鉴定了3个基因型。测序揭示了核苷酸过渡，A2205G（GenBank登录号NC_006092），这是一个同义突变，导致赖氨酸的密码子从AAA变为AAG。基因型GG和AG的蛋壳百分比和断裂强度分别高于AA（ P <0.05）;基因型GG的血清总钙分别比基因型AA和AG高11.8％和10.1％。基因型GG的血清磷酸盐分别比基因型AA和AG大16.7％和12％。所有基因型共享相同的钙：磷酸盐比率。对于血清PTH，基因型GG比基因型AA高约30％。因此，PTH中的SNP A2205G影响了蛋壳百分比和断裂强度，并且可能与血清钙和PTH水平的变化有关，这表明PTH中的SNP具有在MAS程序中用于鸡肉蛋质量的潜力。 。

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