• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Poultry Science >Derivation and characteristics of pluripotent embryonic germ cells in duckp#.
【24h】

Derivation and characteristics of pluripotent embryonic germ cells in duckp#.

机译：鸭多能胚胎生殖细胞的衍生和特征

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Embryonic germ (EG) cells derived from primordial germ cells are undifferentiated and pluripotent stem cells. In our study, primordial germ cells isolated from the genital ridges of Beijing duck (Anas domestica) embryo at stage 28 were co-cultured with mice embryonic fibroblasts. Duck EG cells grew with a high nuclear:cytoplasm ratio and were positive for periodic acid-Schiff and alkaline phosphatase staining. The duck EG cells could proliferate for more than a month in repeated subculture and maintain a diploid karyotype. They expressed the pluripotent markers such as stage-specific embryonic antigen-1, stage-specific embryonic antigen-4, tumor rejection antigen-1-60, and tumor rejection antigen-1-81. Furthermore, the EG cells could form embryoid bodies and differentiate to osteoblast in vitro. This study explored the isolation and culture process of duck EG cells using the culture methods of chicken EG cells and investigated biological characteristics of Beijing duck EG cells. Our results showed that methods published for the culture of chicken EG cells could be applied to the culture of duck EG cells. Duck EG cells will be useful for the differentiation of EG cells in avian species.
机译：源自原始生殖细胞的胚胎生殖（EG）细胞是未分化的多能干细胞。在我们的研究中，从北京鸭（Anas domestica）胚胎在第28阶段的生殖脊分离出的原始生殖细胞与小鼠胚胎成纤维细胞共培养。鸭EG细胞以高的核：细胞质比率生长，并且对高碘酸-希夫和碱性磷酸酶染色呈阳性。鸭的EG细胞在重复的继代培养中可以增殖超过一个月，并保持二倍体核型。他们表达了多能标记，例如阶段特异性胚胎抗原-1，阶段特异性胚胎抗原-4，肿瘤排斥抗原-1-60和肿瘤排斥抗原-1-81。此外，EG细胞可以形成胚状体并在体外分化为成骨细胞。本研究利用鸡EG细胞的培养方法探索了鸭EG细胞的分离和培养过程，并研究了北京鸭EG细胞的生物学特性。我们的结果表明，已公开的培养鸡EG细胞的方法可应用于鸭EG细胞的培养。鸭EG细胞可用于禽类中EG细胞的分化。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号