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首页> 外文期刊>Poultry Science >Allele-specific expression analysis reveals CD79B has a cis-acting regulatory element that responds to Marek's disease virus infection in chickens
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Allele-specific expression analysis reveals CD79B has a cis-acting regulatory element that responds to Marek's disease virus infection in chickens

机译：等位基因特异性表达分析表明CD79B具有顺式作用调控元件，可响应鸡的马立克氏病病毒感染

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Marek's disease (MD) is a T cell lymphoma disease of domestic chickens induced by the Marek's disease virus (MDV), a highly infectious and naturally oncogenic alphaherpesvirus. Enhancing genetic resistance to MD in poultry is an attractive method to augment MD vaccines, which protect against MD but do not prevent MDV replication and horizontal spread. Previous work integrating QTL scans, transcript profiling, and MDV-chicken protein-protein interaction screens revealed 3 MD resistance genes; however, a major challenge continues to be the identification of the other contributing genes. To aid in this search, we screened for allele-specific expression (ASE) in response to MDV infection, a simple and novel method for identifying polymorphic cis-acting regulatory elements, which may contain strong candidate genes with specific alleles that confer MD genetic resistance. In this initial study, we focused on immunoglobulin beta (CD79B) because it plays a critical role in the immune response and, more important, is transcriptionally coupled with growth hormone (GH1), one of the previously identified MD resistance genes. Using a coding SNP in CD79B and pyrosequencing to track the relative expression of each allele, we monitored ASE in uninfected and MDV-infected F-1 progeny from reciprocal intermatings of highly inbred chicken lines 6(3) (MD resistant) and 7(2) (MD susceptible). Upon screening 3 tissues (bursa, thymus, and spleen) at 5 time points (1, 4, 7, 11, and 15 d postinfection), we observed that MDV infection alters the CD79B allelic ratios in bursa and thymus tissues at 4 and 15 d postinfection in both mating directions. Our results suggest that CD79B has a cis-acting regulatory element that responds to MDV infection and probably cooperates with GH1 in conferring genetic resistance to MD. This result helps validates the use of ASE screens to identify specific candidate genes for complex traits such as genetic resistance to MD.
机译：马立克氏病（MD）是由马立克氏病病毒（MDV）引起的家鸡的T细胞淋巴瘤疾病，该病毒是一种高度传染性和天然致癌性α疱疹病毒。提高家禽对MD的遗传抗性是增加MD疫苗的一种有吸引力的方法，该疫苗可预防MD，但不能防止MDV复制和水平传播。整合QTL扫描，转录谱分析和MDV-鸡蛋白-蛋白相互作用筛选的先前工作揭示了3个MD抗性基因。然而，主要的挑战仍然是其他贡献基因的鉴定。为帮助进行此搜索，我们筛选了针对MDV感染的等位基因特异性表达（ASE），这是一种用于识别多态性顺式调控元件的简单新颖方法，其中可能包含具有特定等位基因的强候选基因，这些基因赋予了MD遗传抗性。在这项初步研究中，我们集中于免疫球蛋白beta（CD79B），因为它在免疫应答中起关键作用，更重要的是，它与先前确定的MD抗性基因之一的生长激素（GH1）转录结合。使用CD79B中的编码SNP并进行焦磷酸测序来跟踪每个等位基因的相对表达，我们从高度近亲鸡品系6（3）（MD耐性）和7（2）的互作中监测了未感染和MDV感染的F-1后代中的ASE ）（易感染MD）。在5个时间点（感染后1、4、7、11和15 d）筛查3个组织（囊，胸腺和脾脏）后，我们观察到MDV感染改变了囊肿和胸腺组织在4和15时CD79B等位基因比率d两个交配方向的感染后。我们的结果表明，CD79B具有顺式作用的调控元件，可响应MDV感染，并且可能与GH1协同作用，赋予对MD的遗传抗性。该结果有助于验证使用ASE筛选来鉴定复杂性状（例如对MD的遗传抗性）的特定候选基因。

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