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首页> 外文期刊>Poultry Science >Quantitative trait loci associated with the humoral innate immune response in chickens were confirmed in a cross between Green-Legged Partridgelike and White Leghorn.
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Quantitative trait loci associated with the humoral innate immune response in chickens were confirmed in a cross between Green-Legged Partridgelike and White Leghorn.

机译：在绿腿Part样和白色来亨鸡的杂交中证实了与鸡体液先天免疫反应相关的数量性状基因座。

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摘要

Natural antibodies (NA) create a crucial barrier at the initial steps of the innate humoral immune response. The main role of NA in the defense system is to bind the pathogens at early stages of infection. Different pathogens are recognized by the presence of highly conserved antigen determinant [e.g., lipopolysaccharide (LPS) in gram-negative bacteria or lipoteichoic acid (LTA) in gram-positive bacteria]. In chickens, a different genetic background of NA binds LPS and LTA antigens, encoded by different QTL. The main objective of this work was to confirm known QTL associated with LPS and LTA NA. For this purpose a chicken reference population was created by crossing 2 breeds: a commercial layer, White Leghorn, and a Polish indigenous chicken, Green-Legged Partridgelike. The chromosomal regions analyzed harbored to GGA3, GGA5, GGA6, GGA8, GGA9, GGA10, GGA14, GGA15, GGA18, and GGAZ. The data collected consisted of the NA titers binding LPS and LTA (determined by ELISA at 12 wk of age) as well as the genotypes (30 short tandem repeat markers; average of 3 markers/chromosome, collected for generations F(0), F(1), and F(2)). The analyses were performed with 3 statistical models (paternal and maternal half-sib, line cross, and linkage analysis and linkage disequilibrium) implemented in GridQTL software (http://www.gridqtl.org.uk/). The QTL study of humoral innate immune response traits resulted in the confirmation of 3 QTL associated with NA titers binding LPS (located on GGA9, GGA18, and GGAZ) and 2 QTL associated with NA titers binding LTA (located on GGA5 and GGA14). A set of candidate genes within the regions of the validated QTL has been proposed.
机译：天然抗体（NA）在先天体液免疫应答的初始步骤中形成了重要的屏障。 NA在防御系统中的主要作用是在感染的早期阶段结合病原体。通过高度保守的抗原决定簇[例如，革兰氏阴性菌中的脂多糖（LPS）或革兰氏阳性菌中的脂蛋白酸（LTA）]识别不同的病原体。在鸡中，NA的不同遗传背景会结合由不同QTL编码的LPS和LTA抗原。这项工作的主要目的是确认与LPS和LTA NA相关的已知QTL。为此目的，通过杂交两个品种创建了一个参考鸡群：一个商业层，白色来亨鸡，和一个波兰本土鸡，绿色腿Part。分析的染色体区域包含GGA3，GGA5，GGA6，GGA8，GGA9，GGA10，GGA14，GGA15，GGA18和GGAZ。收集的数据包括结合LPS和LTA的NA效价（通过ELISA在12周龄时确定）以及基因型（30个短串联重复标记;平均3个标记/染色体，针对F（0），F代收集） （1）和F（2））。使用GridQTL软件（http://www.gridqtl.org.uk/）中实现的3种统计模型（父亲和母亲半同胞，线交叉以及连锁分析和连锁不平衡）进行了分析。体液先天免疫反应性状的QTL研究导致确认了3个与NA滴度结合LPS相关的QTL（位于GGA9，GGA18和GGAZ）和2个与NA滴度结合LTA相关的QTL（位于GGA5和GGA14）。已经提出了在经验证的QTL区域内的一组候选基因。

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