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首页> 外文期刊>Poultry Science >Effect of manipulation of incubation temperature on fatty acid profiles and antioxidant enzyme activities in meat-type chicken embryos.
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Effect of manipulation of incubation temperature on fatty acid profiles and antioxidant enzyme activities in meat-type chicken embryos.

机译：孵育温度的控制对肉型鸡胚脂肪酸组成和抗氧化酶活性的影响。

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Eggs (n=1,800) obtained from Ross broiler breeders at 32 and 48 wk of age were incubated at either a constant temperature of 37.6 degrees C throughout (T1), or the temperature was reduced for 6 h to 36.6 degrees C each day during embryonic age (EA) 10 to 18 (T2). Yolk sac, liver, and brain fatty acid profiles and oxidant and antioxidant status of liver and brain were measured at EA 14, 19, and day of hatch (DOH). Fatty acid profiles of yolk sac, liver, and brain were influenced by age of breeder with significant breeder hen age x incubation temperature interactions. At EA 14, higher levels of 20:4n-6 and 22:6n-3 had been transferred from the yolk sac to T2 embryos from younger than older breeders, whereas for T1 and T2 embryos, yolk sac 20:4n-6 and 22.6n-3 values were similar for older breeders. Accumulation of 20:4n-6 and 22:6n-3 fatty acids in the liver of T1 and T2 embryos from younger breeders was similar; however, T2 embryos from older breeders had higher liver levels of 20:4n-6 and 22:6n-3 than T1 embryos. At EA 19, liver nitric oxide levels were higher for T2 embryos from younger breeders than those from breeders incubated at T1. Brain catalase levels of T2 embryos from younger breeders were higher than those from older breeders at DOH. Thus, changes in fatty acid profiles and catalase and nitric oxide production of brain and liver tissues resulting from 1 degrees C lower incubation temperature from EA 10 to 18 reflect adaptive changes.
机译：从32周龄和48周龄的罗斯肉鸡种鸡那里获得的卵（n = 1,800）在整个温度（T1）内在37.6摄氏度的恒定温度下孵育，或者在胚胎发育期间每天将温度降低6小时至36.6摄氏度年龄（EA）10到18（T2）。在EA 14、19和孵化日（DOH）分别测量卵黄囊，肝脏和大脑的脂肪酸分布以及肝脏和大脑的氧化剂和抗氧化剂状态。卵黄囊，肝脏和大脑的脂肪酸谱受育种年龄的影响，育种年龄与孵化温度之间存在显着关系。在EA 14处，从年幼的种鸡开始，卵黄囊中的20：4n-6和22：6n-3的水平更高，已从卵黄囊转移到T2胚胎，而对于T1和T2胚胎，卵黄囊20：4n-6和22.6 n-3值与年长的繁殖者相似。年轻种鸡的T1和T2胚胎肝脏中20：4n-6和22：6n-3脂肪酸的积累是相似的。但是，来自较早育种者的T2胚胎的肝脏水平比T1胚胎高20：4n-6和22：6n-3。在EA 19，年轻育种者的T2胚胎的肝一氧化氮水平高于在T1孵化的育种者的T2胚胎。在DOH，年轻育种者T2胚胎的脑过氧化氢酶水平高于老年育种者。因此，从EA 10到18降低1摄氏度的孵化温度会导致大脑和肝脏组织的脂肪酸分布以及过氧化氢酶和一氧化氮产生的变化，反映了适应性变化。

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