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首页> 外文期刊>Poultry Science >Expression analysis of global gene response to chronic heat exposure in broiler chickens (Gallus gallus) reveals new reactive genes
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Expression analysis of global gene response to chronic heat exposure in broiler chickens (Gallus gallus) reveals new reactive genes

机译：肉鸡（Gallus gallus）对慢性热暴露的全球基因反应的表达分析揭示了新的反应性基因

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摘要

The process of heat regulation is complex and the exact molecular mechanism is not fully understood. To investigate the global gene response to chronic heat exposure, a breast muscle cDNA library and a liver tissue cDNA library from Silkie fowl were constructed and analyzed in bioinformatics. A total of 8,935 nonredundant EST were identified from and used for gene expression analysis. Microarray assay revealed that in breast muscle of broiler chickens (Gallus gallus), 110 genes changed expression levels after 3 wk of cycling heat stress. Ubiquitin B (UBB); ubiquitin C (UBC); tumor necrosis factor receptor-associated factor 3-interacting Jun amino-terminal kinase activating modulator (TRAF3IP3); eukaryotic translation initiation factor 3, subunit 6 (EIF3S6); poly(A) binding protein, cytoplasmic 1 (PABPC1); and F-box only protein 11 (FBXO11) were the only genes that have been reported to be involved in heat regulation; the majority of the other genes were shown to be related for the first time. The finding of new heat-reactive genes left perpendicularmitogen-activated protein kinase activating protein PM20/PM21; suppressors of cytokine signaling (SOCS) box-containing protein 2 (ASB2); ubiquitin-specific proteinase 45 (USP45); and TRK-fused gene (TFG)right perpendicular suggests that the mitogen-activated protein kinase pathways as well as the ubiquitin-proteasome pathways and the nuclear factor kappa B pathways play important roles in heat regulation. This study provides new information on the regulation of heat stress, though the mechanism is far from being understood. Further in-depth research on the newly discovered heat-reactive genes is required to fully understand their molecular functions in thermoregulation.
机译：热调节的过程很复杂，确切的分子机理尚不完全清楚。为了研究对慢性热暴露的总体基因反应，构建了来自Silkie Fowl的乳房肌肉cDNA文库和肝脏组织cDNA文库，并进行了生物信息学分析。从基因表达分析中鉴定出总共8,935个非冗余EST，并将其用于基因表达分析。基因芯片检测显示，在3周的热应激循环中，肉鸡（鸡）的胸肌中有110个基因改变了表达水平。泛素B（UBB）;泛素C（UBC）;与肿瘤坏死因子受体相关的因子3相互作用的Jun氨基末端激酶激活调节剂（TRAF3IP3）;真核翻译起始因子3，亚基6（EIF3S6）;聚（A）结合蛋白，细胞质1（PABPC1）;据报道，仅有F盒蛋白11（FBXO11）参与热量调节。其他大多数基因首次显示出相关性。发现新的热反应基因，使垂直丝裂原激活的蛋白激酶激活蛋白PM20 / PM21离开;细胞因子信号传导（SOCS）盒蛋白2（ASB2）的抑制剂;泛素特异性蛋白酶45（USP45）;与TRK融合基因（TFG）的垂直关系表明，丝裂素激活的蛋白激酶途径以及泛素-蛋白酶体途径和核因子kappa B途径在热量调节中起重要作用。这项研究提供了有关热应力调节的新信息，尽管其机理尚不清楚。为了充分了解它们在温度调节中的分子功能，需要对新发现的热反应基因进行进一步的深入研究。

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