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首页> 外文期刊>Poultry Science >Identification and quantification of aflatoxins and aflatoxicol from poultry feed and their recovery in poultry litter.
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Identification and quantification of aflatoxins and aflatoxicol from poultry feed and their recovery in poultry litter.

机译:鉴定和定量家禽饲料中的黄曲霉毒素和黄曲霉毒素及其在家禽垫料中的回收率。

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Aflatoxins (AF) are toxic fungal secondary metabolites and are known mycotoxins pathological to animals and humans. Poultry litter is frequently used as a food supplement for ruminants, and when poultry feed contains AF, the litter becomes contaminated as well, thus having an effect on livestock health. This study identified and quantified AF (AFB, AFB, AFG, and AFG) from poultry feed and their recovery, together with their metabolites (AFM, AFM, AFP, and aflatoxicol) in litter. An experiment with 25 Hy-Line W-36 hens, in their second production stage, 121 wk old, was carried out. Hens were distributed in 3 groups placed in individual cages and 1 ration of 250 g of feed was given to each hen daily. Nine hens of the control group were fed with clean feed, without AFB; the other 2 experimental groups, with 8 hens each, were fed with 2 AFB concentrations: 30 and 500 ogp"kgp#. The feed was replaced and weighed daily throughout a 7-d period to register the amount of feed consumed by the hens. Litter from each hen was collected, weighed, and dried individually. The chemical analysis of 40 g of each one of the 200 feed and 200 litter samples was chemically extracted and concentrated with immunoaffinity columns for total AF. To quantify AF, calibration curves for each AF were done by HPLC. Feed samples of the 3 groups presented significant difference with AFB and AFG, whereas in litter samples, there were significant differences for AFG in the 500 ogp"kgp# group. Poultry litter had traces of AFM, AFM, AFP, and AFL with no significant differences among treatments. Aflatoxin B prevalence in litter samples can cause damages in livestock because this mycotoxin reduces the digestibility of ruminant feed up to 67%.
机译:黄曲霉毒素(AF)是有毒的真菌次生代谢产物,是已知对动物和人类有害的真菌毒素。家禽垫料经常被用作反刍动物的食品补充剂,当家禽饲料中含有AF时,垫料也会被污染,从而影响牲畜的健康。这项研究鉴定并定量了家禽饲料中的AF(AFB,AFB,AFG和AFG)及其回收,以及其垫料中的代谢产物(AFM,AFM,AFP和黄曲霉酚)。在第二个生产阶段(121周龄)对25头Hy-Line W-36母鸡进行了实验。将母鸡分成三组,分别放在单独的笼子里,每天给每只母鸡配饲1份250克饲料。对照组9只母鸡饲喂纯净饲料,无AFB。其他2个实验组(每组8只母鸡)分别饲喂2种AFB浓度:30和500 ogp“ kgp#。在7天的时间内每天更换饲料并称重,以记录母鸡消耗的饲料量。收集每只母鸡的凋落物,称重并干燥,分别化学提取200份饲料和200磅样品中的每份40 g的化学分析,并用免疫亲和柱进行浓缩以得到总AF。通过HPLC进行AF,3组饲料样品与AFB和AFG存在显着差异,而在垫料样品中,500 ogp“ kgp#组的AFG存在显着差异。家禽垫料具有AFM,AFM,AFP和AFL的痕迹,各处理之间无显着差异。垃圾样品中的黄曲霉毒素B患病率可能对牲畜造成损害,因为这种霉菌毒素将反刍动物饲料的消化率降低了67%。

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