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首页> 外文期刊>Poultry Science >Validation of a spectrophotometer-based method for estimating daily sperm production and deferent duct transit.
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Validation of a spectrophotometer-based method for estimating daily sperm production and deferent duct transit.

机译：基于分光光度计的方法的有效性，该方法可用于估计每日精子产生和不同的导管运输。

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The objectives of the present work were 3-fold. First, a new method for estimating daily sperm production was validated. This method, in turn, was used to evaluate testis output as well as deferent duct throughput. Next, this analytical approach was evaluated in 2 experiments. The first experiment compared left and right reproductive tracts within roosters. The second experiment compared reproductive tract throughput in roosters from low and high sperm mobility lines. Standard curves were constructed from which unknown concentrations of sperm cells and sperm nuclei could be predicted from observed absorbance. In each case, the independent variable was based upon hemacytometer counts, and absorbance was a linear function of concentration. Reproductive tracts were excised, semen recovered from each duct, and the extragonadal sperm reserve determined by multiplying volume by sperm cell concentration. Testicular sperm nuclei were procured by homogenization of a whole testis, overlaying a 20-mL volume of homogenate upon 15% (wt/vol) Accudenz (Accurate Chemical and Scientific Corporation, Westbury, NY), and then washing nuclei by centrifugation through the Accudenz layer. Daily sperm production was determined by dividing the predicted number of sperm nuclei within the homogenate by 4.5 d (i.e., the time sperm with elongated nuclei spend within the testis). Sperm transit through the deferent duct was estimated by dividing the extragonadal reserve by daily sperm production. Neither the efficiency of sperm production (sperm per gram of testicular parenchyma per day) nor deferent duct transit differed between left and right reproductive tracts (P>0.05). Whereas efficiency of sperm production did not differ (P>0.05) between low and high sperm mobility lines, deferent duct transit differed between lines (P<0.001). On average, this process required 2.2 and 1.0 d for low and high lines, respectively. In summary, we developed and then tested a method for quantifying male reproductive tract throughput. This method makes the study of semen production amenable to systems biology.
机译：当前工作的目标是三方面。首先，验证了估计每日精子产量的新方法。该方法又用于评估睾丸的输出以及不同的导管通量。接下来，在2个实验中评估了这种分析方法。第一个实验比较了公鸡内的左右生殖道。第二个实验比较了来自低和高精子活动性系的公鸡的生殖道通量。建立标准曲线，从该曲线可以从观察到的吸光度预测未知浓度的精子细胞和精子核。在每种情况下，独立变量均基于血细胞计数器的计数，吸光度是浓度的线性函数。切除生殖道，从每个导管中回收精液，并通过将体积乘以精子细胞浓度来确定性腺外精子储备。睾丸精子核通过整个睾丸的均质化获得，在15％（wt / vol）Accudenz（Accurate Chemical and Scientific Corporation，Westbury，NY）上覆盖20 mL体积的匀浆，然后通过Accudenz离心来洗涤细胞核层。通过将匀浆中的精子核的预测数目除以4.5 d（即精子在睾丸内具有伸长的核的时间）来确定每日精子的产生。通过将性腺外储备除以每日精子产量来估算精子通过输精管的转运。左，右生殖道之间的精子生产效率（每天每克睾丸薄壁组织中的精子）和不同的输精管输送率均无差异（P> 0.05）。低和高精子迁移率系之间的精子生产效率没有差异（P> 0.05），而不同系之间的不同的导管输送差异（P <0.001）。平均而言，低压和高压管线该过程分别需要2.2 d和1.0 d。总而言之，我们开发并测试了量化男性生殖道通量的方法。这种方法使精液生产的研究适合系统生物学。

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