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首页> 外文期刊>Poultry Science >Salmonella enterica serovar Typhimurium utilizes the ClpPX and Lon proteases for optimal fitness in the ceca of chickens
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Salmonella enterica serovar Typhimurium utilizes the ClpPX and Lon proteases for optimal fitness in the ceca of chickens

机译：肠炎沙门氏菌鼠伤寒沙门氏菌利用ClpPX和Lon蛋白酶在鸡盲肠中获得最佳适应性

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Salmonella enterica serovar Typhimurium (S. Typhimurium) is a leading cause of salmonellosis. Poultry and poultry products are implicated in transmission of Salmonella to humans. In 2013, an outbreak of S. Typhimurium occurred that comprised 39 states within the United States and was associated with backyard flocks of chickens. Colonization of the avian host by S. Typhimurium requires numerous genetic factors encoded within the bacterium. Of particular interest are genetic factors induced by alternative sigma factors within S. Typhimurium since these genetic elements are important for adaptation to different environmental stresses. The heat shock response is a dedicated change in gene regulation within bacteria in response to several stresses, specifically growth at 42 degrees C. Because chickens have a higher body temperature than other animals (42 degrees C) the hypothesis was tested that components of the heat shock response are important for optimal fitness within the chicken. To this end, deletion of the heat shock proteases clpPX (BTNC0022) or lon (BTNC0021) was accomplished and the bacterial fitness in vivo was compared to the "wild-type" strain (NC1040) using a competition assay. One-day-old chicks were orally gavaged with an equal mixture of NC1040 and either BTNC0022 or BTNC0021. Quantification of viable bacteria over time by using plate counts indicated that deletion of either heat shock protease resulted in significantly reduced colonization of the chicken ceca compared to the wildtype strain. To satisfy the molecular Koch's postulates, clpPX and lon mutants were complemented in trans using a low- copy number plasmid for additional in vivo experiments. Complementation studies confirmed the importance of either heat shock protease to colonization of the chicken ceca. This report demonstrated that both ClpPX and Lon were important for optimal fitness within chickens. Moreover, these results suggested that components of the heat shock may be critical factors used by S. Typhimurium for colonization of poultry. The use of feed additives or other treatments that inactivate or inhibit ClpPX or Lon may reduce the bacterial burden of S. Typhimurium in poultry.
机译：肠炎沙门氏菌鼠伤寒沙门氏菌（S. Typhimurium）是沙门氏菌病的主要原因。家禽和家禽产品与沙门氏菌向人类的传播有关。 2013年，爆发了伤寒沙门氏菌，在美国39个州内发生，并与后院鸡群有关。鼠伤寒沙门氏菌对鸟类宿主的定殖需要细菌内编码的许多遗传因子。特别令人感兴趣的是由鼠伤寒沙门氏菌中的另一些σ因子诱导的遗传因子，因为这些遗传元素对于适应不同的环境压力很重要。热休克反应是细菌对多种压力（特别是在42摄氏度下生长）的反应中细菌内基因调控的专门变化。由于鸡的体温高于其他动物（42摄氏度），因此对假设进行了检验休克反应对于鸡的最佳适应性很重要。为此，完成了热休克蛋白酶clpPX（BTNC0022）或lon（BTNC0021）的缺失，并且使用竞争测定法将体内细菌适应性与“野生型”菌株（NC1040）进行了比较。将1天大的雏鸡口服NC1040和BTNC0022或BTNC0021的等量混合物。通过使用平板计数对存活细菌进行定量，表明与野生型菌株相比，两种热休克蛋白酶的缺失均导致鸡盲肠定植明显减少。为了满足分子的Koch假设，使用低拷贝数质粒反式补充了clpPX和lon突变体用于其他体内实验。补充研究证实了两种热激蛋白酶对鸡盲肠定植的重要性。该报告表明，ClpPX和Lon均对鸡的最佳适应性很重要。此外，这些结果表明，热激的成分可能是鼠伤寒沙门氏菌用于家禽定殖的关键因素。使用饲料添加剂或其他能灭活或抑制ClpPX或Lon的处理方法可以减少家禽伤寒沙门氏菌的细菌负担。

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