• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Plasmid: An International Journal Devoted to Extrachromosomal Gene Systems >Characterization of a new mitochondrial plasmid from Fusarium proliferatum.
【24h】

Characterization of a new mitochondrial plasmid from Fusarium proliferatum.

机译：镰刀菌新线粒体质粒的表征。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

A 10.3kb linear mitochondrial DNA plasmid designated pFP1 was isolated from Fusarium proliferatum. The DNA sequence of the plasmid consists of 10,336bp with perfect terminal inverted repeats of 400bp. Two major, non-overlapping ORFs were identified on opposite strands, encoding a phage-type RNA polymerase and a family B type DNA polymerase, respectively. One additional minor ORF encoding a putative highly basic protein was also identified. The copy number of pFP1, as determined by RT-PCR, ranged between 1.8 and 3.1 per mtDNA copies depending on the host strain. Real-time PCR analysis of a total of 400 cultures surviving ethidium bromide curing indicated that no plasmid-free strains could be obtained by this treatment. Further single spore selections of the survivors with reduced plasmid content were needed to obtain plasmid-free clones. No phenotypic differences were found between the wild-type strains and their plasmid-free progenies.
机译：从枯萎镰刀菌中分离出10.3kb的线性线粒体DNA质粒，命名为pFP1。质粒的DNA序列由10,336bp组成，具有完美的末端反向重复序列400bp。在相反的链上鉴定出两个主要的，不重叠的ORF，分别编码噬菌体型RNA聚合酶和B族DNA聚合酶。还鉴定了另一种编码假定的高度碱性蛋白的次要ORF。通过RT-PCR确定，pFP1的拷贝数在mtDNA拷贝数1.8到3.1之间，具体取决于宿主菌株。实时PCR分析了总共400种在溴乙锭固化后仍存活的培养物，结果表明该处理无法获得不含质粒的菌株。为了获得无质粒的克隆，还需要对具有减少的质粒含量的幸存者进行进一步的单孢子选择。在野生型菌株及其无质粒后代之间未发现表型差异。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号