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首页> 外文期刊>Plasmid: An International Journal Devoted to Extrachromosomal Gene Systems >The complete sequence and functional analysis of pANL, the large plasmid of the unicellular freshwater cyanobacterium Synechococcus elongatus PCC 7942.
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The complete sequence and functional analysis of pANL, the large plasmid of the unicellular freshwater cyanobacterium Synechococcus elongatus PCC 7942.

机译：pANL的完整序列和功能分析，pANL是单细胞淡水蓝藻细长突触球菌PCC 7942的大质粒。

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摘要

Two endogenous plasmids are present in Synechococcus elongatus PCC 7942, a model organism for studying photosynthesis and circadian rhythms in cyanobacteria. The large plasmid, pANL, was shown previously to be involved in adaptation of S. elongatus cells to sulfur starvation, which provided the first evidence of cellular function of a cyanobacterial plasmid. Here, we report the complete sequence of pANL, which is 46,366 bp in length with 53% GC content and encodes 58 putative ORFs. The pANL plasmid can be divided into four structural and functional regions: the replication origin region, a signal transduction region, a plasmid maintenance region, and a sulfur-regulated region. Cosmid-based deletion analysis suggested that the plasmid maintenance and replication origin regions are required for persistence of pANL in the cells. Transposon-mediated mutagenesis and complementation-based pANL segregation assays confirmed that two predicted toxin-antitoxin cassettes encoded in the plasmid maintenance region, belonging to PemK and VapC families, respectively, are necessary for plasmid exclusion. The compact and efficient organization of sulfur-related genes on pANL may provide selective advantages in environments with limited sulfur.
机译：伸长的聚球藻PCC 7942中存在两个内源质粒，这是一种用于研究蓝细菌中的光合作用和昼夜节律的模型生物。先前显示大质粒pANL参与了伸长链球菌细胞对硫缺乏的适应，这为蓝细菌质粒的细胞功能提供了第一个证据。在这里，我们报告了pANL的完整序列，全长46,366 bp，GC含量为53％，编码58个假定的ORF。 pANL质粒可分为四个结构和功能区：复制起点区，信号转导区，质粒维持区和硫调节区。基于粘粒的缺失分析表明质粒维持和复制起点区域是细胞中pANL持久性所必需的。转座子介导的诱变和基于互补的pANL分离试验证实，质粒排除区域中分别需要两个预测的毒素-抗毒素盒，分别在质粒维持区中编码，分别属于PemK和VapC家族。 pANL上与硫有关的基因的紧凑高效组织可以在硫含量有限的环境中提供选择性优势。

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