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首页> 外文期刊>Plasmid: An International Journal Devoted to Extrachromosomal Gene Systems >Complete nucleotide sequence of pGO1, the prototype conjugative plasmid from the Staphylococci.
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Complete nucleotide sequence of pGO1, the prototype conjugative plasmid from the Staphylococci.

机译：pGO1的完整核苷酸序列，是葡萄球菌的原型结合质粒。

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摘要

In view of its historical significance as the prototype class III plasmid from the staphylococci, and its ongoing importance as a laboratory tool, we have determined the complete nucleotide sequence of pGO1. At exactly 54kb, pGO1 is 2-4kb larger than previously reported, and shares extensive ( approximately 31-46kb) regions of near identical DNA sequence with other class III plasmids. In particular, we confirm that pGO1 is almost identical to plasmid pSK41 along the entire length of the latter, but additionally contains a co-integrated copy of plasmid pSK639, which accounts for the difference in size ( approximately 8kb), and the fact that pGO1, but not pSK41, confers resistance to trimethoprim. The pSK639 co-integrant appeared to have undergone mutational inactivation of its mobilization functions, a finding which was confirmed experimentally. Although originally identified through an association with aminoglycoside resistance, the pGO1/pSK41 backbone replicon continues to play a key role in the dissemination of antibiotic resistance determinants in the staphylococci.
机译：鉴于其作为葡萄球菌原型III类质粒的历史意义以及作为实验室工具的持续重要性，我们确定了pGO1的完整核苷酸序列。 pGO1正好是54kb，比以前报道的大了2-4kb，并与其他III类质粒共享了几乎相同的DNA序列的广泛区域（大约31-46kb）。特别是，我们确认pGO1在质粒pSK41的整个长度上几乎与质粒pSK41相同，但另外包含质粒pSK639的共整合拷贝，这解释了大小的差异（大约8kb），以及pGO1而非pSK41赋予了对甲氧苄啶的抗药性。 pSK639共整合物似乎对其动员功能发生了突变失活，这一发现已通过实验得到证实。尽管最初是通过与氨基糖苷耐药性的关联来鉴定的，但pGO1 / pSK41骨架的复制子在葡萄球菌中的抗生素耐药性决定因素的传播中仍起着关键作用。

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