Isolation and sequence analysis of a small cryptic plasmid pRK10 from a corrosion inhibitor degrading strain Serratia marcescens ACE2.

Syed Ibrahim K; Bakkiyaraj D; James R

首页> 外文期刊>Plasmid: An International Journal Devoted to Extrachromosomal Gene Systems >Isolation and sequence analysis of a small cryptic plasmid pRK10 from a corrosion inhibitor degrading strain Serratia marcescens ACE2.

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Isolation and sequence analysis of a small cryptic plasmid pRK10 from a corrosion inhibitor degrading strain Serratia marcescens ACE2.

机译：来自腐蚀抑制剂降解菌株粘质沙雷氏菌ACE2的小型隐性质粒pRK10的分离和序列分析。

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The nucleotide sequence of a smallest cryptic plasmid pRK10 of Serratia marcescens ACE2 was determined. When compared to the all other plasmids reported so far from S. marcescens in sizes of over 70kb, pRK10 is only 4241bp long with 53% G+C content and has five coding sequences representing a coding percentage of 65.41. This small plasmid consists of one Tdh gene, four mobilization genes, mobCABD, and an origin of replication homologous to those of ColE1-type plasmids. Analysis of the five open reading frames identified on the plasmid suggests the presence of genes involved in replication and mobilization containing sequences homologous to the bom region and mobCABD genes of ColE1 and Tdh from Acinetobacter baumannii str. AYE. Results also indicate that pRK10 does not encode any gene for antibiotic/heavy metal resistance. Copy number and incompatibility of the plasmid with plasmids of ColE1 origin of replication was determined and it is quite stable in its natural host as well as in Escherichia coli DH5alpha. This relatively small plasmid will be useful for construction of shuttle vectors to facilitate the genetic analysis.

机译：测定了粘质沙雷氏菌ACE2的最小隐性质粒pRK10的核苷酸序列。当与迄今报道的距离marcescens S. marcescens的所有其他质粒进行比较时，pRK10的长度仅为4241bp，G + C含量为53％，并且有五个编码序列，代表编码百分比为65.41。这个小质粒由一个Tdh基因，四个动员基因，mobCABD和与ColE1型质粒同源的复制起点组成。对在质粒上鉴定的五个开放阅读框的分析表明，存在与复制和动员有关的基因，该基因包含与鲍曼不动杆菌的ColE1和Tdh的bom区和mobCABD基因同源的序列。好的结果还表明，pRK10不编码任何抗生素/重金属抗性基因。确定了质粒的拷贝数和与ColE1复制起点质粒的不相容性，并且在其天然宿主以及大肠杆菌DH5alpha中非常稳定。该相对较小的质粒将用于构建穿梭载体以促进遗传分析。

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《Plasmid: An International Journal Devoted to Extrachromosomal Gene Systems》 |2009年第3期|共8页
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Syed Ibrahim K; Bakkiyaraj D; James R;
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1. Isolation and sequence analysis of a small cryptic plasmid pRK10 from a corrosion inhibitor degrading strain Serratia marcescens ACE2. [J] . Syed Ibrahim K, Bakkiyaraj D, James R Plasmid: An International Journal Devoted to Extrachromosomal Gene Systems . 2009,第3期

机译：来自腐蚀抑制剂降解菌株粘质沙雷氏菌ACE2的小型隐性质粒pRK10的分离和序列分析。
2. Isolation, characterization, and sequence analysis of cryptic plasmids from Acinetobacter calcoaceticus and their use in the construction of Escherichia coli shuttle plasmids. [J] . W Minas, D L Gutnick Applied and Environmental Microbiology . 1993,第9期

机译：钙不动杆菌不明质粒的分离，鉴定和序列分析及其在构建大肠杆菌穿梭质粒中的应用。
3. Sequence Analysis of Putative luxS Gene Involved in Prodigiosin Biosynthesis from Philippine Local Strains of Serratia marcescens [J] . KatrinaC. Martinez, FrancoG. Teves, Madamba Ma.ReinaSuzetteB. International Research Journal of Biological Sciences . 2013,第4期

机译：菲律宾粘质沙雷氏菌本地菌株原虫合成生物的推定luxS基因的序列分析
4. Nucleotide sequence of a small cryptic plasmid from Acidithiobacillus ferrooxidans strain A-6 [C] . F. F. Roberto International Biohydrometallurgy Symposium, IBS-2001 Sep 16-19, 2001, Ouro Preto, Minas Gerais, Brazil . 2001

机译：铁氧化酸硫硫杆菌菌株A-6的一个小型隐性质粒的核苷酸序列
5. Arbitrarily primed PCR of cDNA for investigation of differential presence and expression of genes in three lines of Spiroplasma citri, and, Sequence analysis of Serratia marcescens strains associated with cucurbit yellow vine disease. [D] . Rascoe, John Edward. 2000

机译：cDNA的任意引物PCR，用于研究三株柠檬螺旋体中基因的差异性存在和表达，以及与葫芦科黄葡萄病相关的粘质沙雷氏菌菌株的序列分析。
6. Isolation characterization and sequence analysis of cryptic plasmids from Acinetobacter calcoaceticus and their use in the construction of Escherichia coli shuttle plasmids. [O] . W Minas, D L Gutnick 1993

机译：钙不动杆菌不明质粒的分离鉴定和序列分析及其在构建大肠杆菌穿梭质粒中的应用。
7. Plasmid-mediated dissemination of the metallo-beta-lactamase gene blaIMP among clinically isolated strains of Serratia marcescens. [O] . Ito, H, Arakawa, Y, Ohsuka, S, 1995

机译：在临床分离的粘质沙雷氏菌菌株中，质粒介导的金属β-内酰胺酶基因blaIMP的传播。

Isolation and sequence analysis of a small cryptic plasmid pRK10 from a corrosion inhibitor degrading strain Serratia marcescens ACE2.

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