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首页> 外文期刊>Plasmid: An International Journal Devoted to Extrachromosomal Gene Systems >Genetic characterization of pAsa6, a new plasmid from Aeromonas salmonicida subsp. salmonicida that encodes a type III effector protein AopH homolog.
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Genetic characterization of pAsa6, a new plasmid from Aeromonas salmonicida subsp. salmonicida that encodes a type III effector protein AopH homolog.

机译：pAsa6的遗传特性，这是一种来自鲑鱼气单胞菌亚种的新质粒。编码III型效应蛋白AopH同源物的鲑鱼。

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A new plasmid designated pAsa6 from an Aeromonas salmonicida subsp. salmonicida strain isolated from diseased turbot has been characterized. pAsa6 consists of 18536bp, has a G+C content of 53.8% and encodes 20 predicted open-reading frames (ORFs). Eight ORFs showed homology to transposases, of which six are complete and two are partial IS sequences. Two ORFs showed homology to replication proteins, and six ORFs showed homology to hypothetical proteins. Two ORFs are truncated homologs of putative A. salmonicida sulfatases. Two genes, aopH and sycH encode homologs of an effector protein for which a role in fish colonization by A. salmonicida has been previously reported, and its chaperone, respectively. The results of filter conjugation experiments suggested that pAsa6 is not mobilizable, as it failed to be conjugally-transferred to several species of marine bacteria tested. All the ORFs of pAsa6 with the exception of four copies of a IS1 transposase gene, have a counterpart in the recently sequenced 155-kb A. salmonicida plasmid pAsa5, suggesting either that pAsa6 is a derivative of pAsa5, or that pAsa5 is the result of the fusion of a pAsa6-like plasmid and a larger plasmid of ca. 135-kb. The pAsa6-encoded repA and aopH genes could be PCR-amplified from strains lacking pAsa6, suggesting presence of a large, possibly pAsa5-like plasmid that was not detected on agarose gels, or the existence of chromosome-integrated plasmid sequences. This study demonstrates that genomic locations for the aopH gene different to pAsa5 or pAsa5-like plasmids exist in A. salmonicida.
机译：来自鲑鱼气单胞菌亚种的新质粒命名为pAsa6。从患病的大菱t中分离出的沙门氏菌菌株已得到表征。 pAsa6由18536bp组成，G + C含量为53.8％，编码20个预测的开放阅读框（ORF）。八个ORF显示出与转座酶的同源性，其中六个是完整的，两个是部分IS序列。两个ORF显示与复制蛋白同源，六个ORF显示与假设蛋白同源。两个ORF是假定的鲑鱼曲霉硫酸酯酶的截短同源物。两个基因aopH和sycH分别编码一种效应蛋白的同源物，该蛋白先前已经报道了其在沙门氏菌中定殖在鱼类中的作用及其伴侣。过滤器结合实验的结果表明，pAsa6不能动员，因为它不能通过结合转移到几种测试的海洋细菌中。除四份IS1转座酶基因外，pAsa6的所有ORF在最近测序的155 KB鲑鱼沙门氏菌质粒pAsa5中都有对应物，这表明pAsa6是pAsa5的衍生物，或者pAsa5是pAsa5的结果pAsa6样质粒与ca较大质粒的融合。 135 kb。可以从缺少pAsa6的菌株中PCR扩增pAsa6编码的repA和aopH基因，这表明存在琼脂糖凝胶上未检测到的大的，可能是pAsa5样质粒，或者存在染色体整合质粒序列。这项研究表明，鲑鱼曲霉中存在与pAsa5或pAsa5样质粒不同的aopH基因的基因组位置。

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