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首页> 外文期刊>Plasmid: An International Journal Devoted to Extrachromosomal Gene Systems >The role of PEI structure and size in the PEI/liposome-mediated synergism of gene transfection.
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The role of PEI structure and size in the PEI/liposome-mediated synergism of gene transfection.

机译：PEI结构和大小在PEI /脂质体介导的基因转染协同作用中的作用。

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摘要

Polyethylenimines (PEIs) and cationic liposomes are widely used for nonviral gene delivery. When PEIs have been used alone, the transfection efficiency has been higher for larger or linear than smaller or branched PEIs. We have reported previously that a combination of small PEIs and liposomes results in a potentiation of transfection efficiency in vitro. Here, the role of PEI size and structure in this synergism has been clarified further. Therefore, two structurally different high MW PEIs, i.e. the linear PEI22K and branched PEI25K, were studied in the SMC cells. We found that both linear PEI22K and branched PEI25K resulted in a similar synergism and comparable transfection efficiencies. However, the potentiation for larger PEIs found in the present study was weaker than that for smaller PEIs obtained in our previous studies. In conclusion, our present and previous results demonstrate that the increment of PEI/liposome-mediated gene transfection by different types of PEIs in vitro is a common attribute that is rather associated with their size than the structure. Interestingly, the effect of PEI size seems to be opposite when combined with liposome or given alone, i.e. the small PEIs are more effective when combined and less effective when alone than the larger ones.
机译：聚乙烯亚胺（PEI）和阳离子脂质体被广泛用于非病毒基因的传递。当单独使用PEI时，较大或线性的PEI的转染效率高于较小或分支的PEI。先前我们已经报道过，小PEI和脂质体的组合可增强体外转染效率。在这里，PEI大小和结构在这种协同作用中的作用已进一步阐明。因此，在SMC细胞中研究了两种结构不同的高MW PEI，即线性PEI22K和支链PEI25K。我们发现线性PEI22K和支链PEI25K都产生相似的协同作用和相当的转染效率。但是，本研究中发现的较大PEI的增强作用要弱于我们先前研究中获得的较小PEI的增强作用。总之，我们目前和以前的结果表明，体外通过不同类型的PEI进行PEI /脂质体介导的基因转染的增加是一个共同的属性，与其大小而非结构相关。有趣的是，当与脂质体组合或单独使用时，PEI大小的影响似乎是相反的，即，小PEI组合时效果更好，单独使用时比大PEI效果差。

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