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首页> 外文期刊>Plasmid: An International Journal Devoted to Extrachromosomal Gene Systems >SEQUENCE ANALYSIS OF PLASMID PCC5.2 FROM CYANOBACTERIUM SYNECHOCYSTIS PCC 6803 THAT REPLICATES BY A ROLLING CIRCLE MECHANISM
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SEQUENCE ANALYSIS OF PLASMID PCC5.2 FROM CYANOBACTERIUM SYNECHOCYSTIS PCC 6803 THAT REPLICATES BY A ROLLING CIRCLE MECHANISM

机译：蓝藻轮回机理替代的蓝细菌PCC 6803的质粒PCC5.2序列分析。

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摘要

The cyanobacterium Synechocystis sp. strain PCC 6803 contains several cryptic plasmids of 2.4, 5.2, and about 50 or 100 kbp. The complete nucleotide sequence of the 5.2-kbp plasmid, pCC5.2, has been analyzed and is reported here. This plasmid contains 5214 bp and 53.1% A+T. Six open reading frames, ORFs A-F (encoding peptides of larger than 90 amino acid residues), were located on both strands of pCC5.2. ORF B codes for a potential replication protein containing 971 amino acids, for which there are three homologous proteins encoded by other cyanobacterial plasmids. ORF C encodes a polypeptide of 93 amino acids which shares homologies with products of two ORFs found in the protein database. Counterparts of the products of ORF A, D, E, and F could not be found in the protein data base. Detection of a single-stranded DNA intermediate during replication of pCC5.2 indicates that this plasmid may also replicate by a rolling circle mechanism, as has been reported for pCA2.4 and pCB2.4 from the same strain of Synechocystis (PCC 6803). (C) 1997 Academic Press. [References: 27]
机译：蓝藻集胞藻PCC 6803菌株含有2.4、5.2和约50或100 kbp的几种隐性质粒。已经分析并报道了5.2-kbp质粒pCC5.2的完整核苷酸序列。该质粒含有5214bp和53.1％A + T。六个开放阅读框ORF A-F（编码大于90个氨基酸残基的肽）位于pCC5.2的两条链上。 ORF B编码含有971个氨基酸的潜在复制蛋白，该蛋白有3个由其他蓝细菌质粒编码的同源蛋白。 ORF C编码一个93个氨基酸的多肽，该多肽与蛋白质数据库中两个ORF的产物具有同源性。在蛋白质数据库中找不到ORF A，D，E和F的产品对应物。在pCC5.2复制过程中检测到单链DNA中间体表明，该质粒还可以通过滚环机制复制，正如已经报道了来自同一集囊藻（PCC 6803）的pCA2.4和pCB2.4一样。 （C）1997学术出版社。 [参考：27]

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