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首页> 外文期刊>Plasmid: An International Journal Devoted to Extrachromosomal Gene Systems >GENETIC ANALYSIS OF REGIONS INVOLVED IN REPLICATION AND CADMIUM RESISTANCE OF THE PLASMID PND302 FROM LACTOCOCCUS LACTIS
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GENETIC ANALYSIS OF REGIONS INVOLVED IN REPLICATION AND CADMIUM RESISTANCE OF THE PLASMID PND302 FROM LACTOCOCCUS LACTIS

机译：乳球菌的质粒PND302复制和抗镉区域的遗传分析

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The 8.8-kb Lactococcus lactis plasmid pND302 encodes resistance to cadmium (Cd-R). Regions of pND302 involved in replication and Cd-R were subcloned and sequenced The replication region is localized on a 1.5-kb region and consists of an open reading frame (repB) preceded by a noncoding AT-rich sequence (on) which is highly homologous to lactococcal theta-type replicons. The Cd-R determinant is localized on a 2.9-kb region and encodes putative proteins similar to the Cd2+-specific P-type efflux ATPase (CadA) and the transcriptional regulatory repressor (CadC) identified in Staphylococcus aureus, Bacillus firmus, and Listeria monocytogenes. Similar Cd-R determinants were also detected by PCR in other Cd-R plasmids isolated from different L. lactis strains. (C) 1997 Academic Press. [References: 29]
机译：8.8-kb乳酸乳球菌质粒pND302编码对镉（Cd-R）的抗性。对涉及复制和Cd-R的pND302区域进行亚克隆和测序。复制区域位于1.5 kb区域，由一个开放阅读框（repB）和一个高度同源的非编码AT富序列（on）组成。乳球菌θ型复制子。 Cd-R决定簇位于一个2.9kb区域，并编码类似于金黄色葡萄球菌，坚定芽孢杆菌和单核细胞增生李斯特氏菌中鉴定的Cd2 +特异性P型外排ATPase（CadA）和转录调节阻遏物（CadC）的推定蛋白质。 。还通过PCR在分离自不同乳酸乳球菌菌株的其他Cd-R质粒中检测到相似的Cd-R决定簇。 （C）1997学术出版社。 [参考：29]

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