A POTENTIAL NEW GENE (TCCA) ON INCP PLASMID RK2 AND TRANSPOSON TN1721 - RELATIONSHIP OF ITS PRODUCT TO THE TRWC RELAXASE/HELICASE OF INCW PLASMID R388

Jovanovic OS.; Figurski DP.

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A POTENTIAL NEW GENE (TCCA) ON INCP PLASMID RK2 AND TRANSPOSON TN1721 - RELATIONSHIP OF ITS PRODUCT TO THE TRWC RELAXASE/HELICASE OF INCW PLASMID R388

机译：在INCP质粒RK2和转座子TN1721上的潜在新基因（TCCA）-其产物与INCW质粒R388的TRWC松弛酶/解旋酶的关系

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Computational analysis of the fully sequenced 60-kb genome of broad-host-range IncP alpha plasmid RK2 revealed a previously unreported potential protein-coding sequence, an 80-codon open reading frame (tccA), located in the region between the vegetative origin of replication (oriV) and the tetR gene of the tetracycline resistance determinant. The coding region is also present in the transposon Tn1721 tet region, which is nearly identical to the tet region of RK2. Remarkably, the predicted polypeptide product of the coding region displays 56% identity and 72% similarity with the C-terminal domain of the TrwC relaxase/helicase protein of IncW plasmid R388. (C) 1997 Academic Press. [References: 17]

机译：对宽宿主范围的IncPα质粒RK2的60 kb基因组的全序列基因组进行计算分析，发现先前未报道的潜在蛋白编码序列，即80密码子开放阅读框（tccA），位于植物的营养起源之间。复制（oriV）和四环素抗性决定簇的tetR基因。编码区也存在于转座子Tn1721 tet区，该区与RK2的tet区几乎相同。显着地，编码区的预测多肽产物与IncW质粒R388的TrwC松弛酶/解旋酶蛋白的C末端结构域显示56％的同一性和72％的相似性。（C）1997学术出版社。 [参考：17]

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《Plasmid: An International Journal Devoted to Extrachromosomal Gene Systems》 |1997年第3期|共4页
作者
Jovanovic OS.; Figurski DP.;
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正文语种 eng
中图分类医学遗传学;
关键词
Broad-host-range; Plasmid; Incompatibility group p; Rk2; Incompatibility group w; R388; Transposon; Tn1721; Helicase; Relaxase; Complete nucleotide-sequence; Alpha plasmids; R-factors; Organization; Replication; Resistance; Protein; Region;

机译：宽宿主范围;质粒;不相容性组p;Rk2;不相容性组w;R388;转座子;Tn1721;解旋酶;松弛酶;完整的核苷酸序列;Alpha质粒;R因子;组织;复制;抗性;蛋白质;区域;

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1. A POTENTIAL NEW GENE (TCCA) ON INCP PLASMID RK2 AND TRANSPOSON TN1721 - RELATIONSHIP OF ITS PRODUCT TO THE TRWC RELAXASE/HELICASE OF INCW PLASMID R388 [J] . Jovanovic OS., Figurski DP. Plasmid: An International Journal Devoted to Extrachromosomal Gene Systems . 1997,第3期

机译：在INCP质粒RK2和转座子TN1721上的潜在新基因（TCCA）-其产物与INCW质粒R388的TRWC松弛酶/解旋酶的关系
2. NICKING ACTIVITY OF TRWC DIRECTED AGAINST THE ORIGIN OF TRANSFER OF THE INCW PLASMID R388 [J] . Llosa M., Delacruz F., Grandoso G. Journal of Molecular Biology . 1995,第1期

机译：根据INCW质粒R388的转移原点，TRWC的结网活性
3. Conjugation-independent, site-specific recombination at the oriT of the IncW plasmid R388 mediated by TrwC. [J] . M Llosa, S Bolland, G Grandoso, Journal of bacteriology . 1994,第11期

机译：由TrwC介导的IncW质粒R388的oriT处的偶联非依赖性位点特异性重组。
4. Genetic transfer of IncP,IncQ,IncW plasmids to four thiobacillus ferrooxidans strains by conjugation [C] . Z.Liu, F.Borne, J.Ratouchniak, International biohydrometallurgy symposium;IBS'99 . 1999

机译：通过结合将IncP，IncQ，IncW质粒遗传转移至四株氧化亚铁硫杆菌
5. Computational, genetic and biochemical analysis of broad host-range plasmid *RK2 [D] . Jovanovic, Oliver Svetozar 2002

机译：广泛的宿主范围质粒* RK2的计算，遗传和生化分析
6. General organization of the conjugal transfer genes of the IncW plasmid R388 and interactions between R388 and IncN and IncP plasmids. [O] . S Bolland, M Llosa, P Avila, 1990

机译：IncW质粒R388的接合转移基因的一般组织以及R388与IncN和IncP质粒之间的相互作用。
7. General organization of the conjugal transfer genes of the IncW plasmid R388 and interactions between R388 and IncN and IncP plasmids. [O] . Bolland, S, Llosa, M, Avila, P, 1990

机译：IncW质粒R388的接合转移基因的一般组织以及R388与IncN和IncP质粒之间的相互作用。

A POTENTIAL NEW GENE (TCCA) ON INCP PLASMID RK2 AND TRANSPOSON TN1721 - RELATIONSHIP OF ITS PRODUCT TO THE TRWC RELAXASE/HELICASE OF INCW PLASMID R388

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