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首页> 外文期刊>Plasmid: An International Journal Devoted to Extrachromosomal Gene Systems >CHARACTERIZATION OF THE REPLICATION AND MOBILIZATION REGIONS OF THE MULTIRESISTANCE KLEBSIELLA PNEUMONIAE PLASMID PJHCMW1
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CHARACTERIZATION OF THE REPLICATION AND MOBILIZATION REGIONS OF THE MULTIRESISTANCE KLEBSIELLA PNEUMONIAE PLASMID PJHCMW1

机译：多耐药性肺炎克雷伯菌肺炎衣原体PJHCMW1的复制和移动区域的特征

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摘要

A 2.4-kb EcoRI fragment including the replication and origin of transfer regions of the, Klebsiella pneumoniae multiresistance plasmid pJHCMW1 has been cloned and sequenced. The isolated replication region was sufficient for stable maintenance of the plasmid and shares homology with RNA-regulated replicons. Homology was highest with the replication region of the plasmid p15A. Incompatibility experiments, however, determined that pJHCMW1 is compatible with pACYC177, a plasmid harboring the p15A replicon. Differences in their RNA I nucleotide sequences may account for their compatibility. A mobilization origin was also found in the 2.4-kb EcoRI pJHCMW1 DNA fragment analyzed. Conjugation experiments showed that although non-self-transmissible, the recombinant clone including the 2.4-kb EcoRI, pJHCMW1 fragment could be mobilized in the presence of the helper plasmid pRK2073. (C) 1997 Academic Press. [References: 41]
机译：已经克隆并测序了一个2.4kb的EcoRI片段，包括肺炎克雷伯氏菌多抗质粒pJHCMW1的复制和转移区的起源。分离的复制区足以稳定维持质粒并与RNA调节的复制子具有同源性。质粒p15A的复制区的同源性最高。然而，不相容性实验确定pJHCMW1与带有p15A复制子的质粒pACYC177兼容。它们的RNA I核苷酸序列的差异可能是其相容性的原因。在分析的2.4kb EcoRI pJHCMW1 DNA片段中也发现了动员的起源。缀合实验显示，尽管不是自我传播的，但在辅助质粒pRK2073存在的情况下，包括2.4kb EcoRI，pJHCMW1片段的重组克隆仍可动员。 （C）1997学术出版社。 [参考：41]

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