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首页> 外文期刊>Plasmid: An International Journal Devoted to Extrachromosomal Gene Systems >CLONING AND CHARACTERIZATION OF A HIGH-COPY-NUMBER NOVEL INSERTION SEQUENCE FROM CHEMOLITHOTROPHIC THIOBACILLUS FERROOXIDANS
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CLONING AND CHARACTERIZATION OF A HIGH-COPY-NUMBER NOVEL INSERTION SEQUENCE FROM CHEMOLITHOTROPHIC THIOBACILLUS FERROOXIDANS

机译：嗜酸性嗜酸亚铁硫杆菌的高拷贝数新插入序列的克隆与鉴定

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摘要

Two distinct families of repetitive DNA elements (1.4 and 1.2 kb) were identified from S1 nuclease-treated genomic DNA of four strains of Thiobacillus ferrooxidans. The 1.4-kb fragment hybridized with IST2, an insertion sequence of T. ferrooxidans. The 1.2-kb fragment was cloned and sequenced. The sequence (IST445), 1219 bp in length, with features characteristic of an insertion element, has a terminal inverted repeat of 8 bp, which can be further extended to 23 or 48 bp with 9 and 26 mismatches, respectively. It displays 54.4% identity in 967 nucleotides of overlap with ISAE1 of Alcaligenes eutrophus. The IST445 contains three open reading frames which have codon usage almost similar to 56 different coding genes of T. ferrooxidans. In Southern blots of restricted genomic DNAs probed with IST445, each of the several strains of T. ferrooxidans gives a distinctive fingerprint. IST445 is present in the range of 10-20 copies per genome in the four strains studied. (C) 1997 Academic Press. [References: 16]
机译：从S1核酸酶处理的四株氧化亚铁硫杆菌菌株的基因组DNA中鉴定出两个不同的重复DNA元素家族（1.4和1.2 kb）。 1.4 kb片段与铁氧化三叶草的插入序列IST2杂交。克隆并测序了1.2kb的片段。长度为1219 bp的序列（IST445）具有插入元件的特征，其末端反向重复序列为8 bp，可以进一步延伸至23或48 bp，分别具有9和26个错配。它在与拟南芥的ISAE1重叠的967个核苷酸中显示54.4％的同一性。 IST445包含三个开放阅读框，其密码子用法与铁氧化三叶草的56个不同编码基因几乎相似。在用IST445探测的限制性基因组DNA的Southern印迹中，铁氧化三叶草的几种菌株中的每一种都具有独特的指纹。在研究的四个菌株中，每个基因组中IST445的存在范围为10-20个拷贝。 （C）1997学术出版社。 [参考：16]

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