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首页> 外文期刊>Plasmid: An International Journal Devoted to Extrachromosomal Gene Systems >TRANSFECTION OF ACTINOMYCES SPP. BY GENOMIC DNA OF BACTERIOPHAGES FROM HUMAN DENTAL PLAQUE
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TRANSFECTION OF ACTINOMYCES SPP. BY GENOMIC DNA OF BACTERIOPHAGES FROM HUMAN DENTAL PLAQUE

机译：放线菌属SPP的翻译。人类牙菌斑噬菌体的基因组DNA

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摘要

Bacteriophages that produced turbid or clear zones of lysis in strains of Actinomyces were isolated from 22 of 124 samples of fresh human dental:plaque. All human and nonhuman strains of Actinomyces viscosus or Actinomyces naeslundii tested in this study were sensitive to infection by one or more of these phages. In contrast, none of the Actinomyces odontolyticus, Actinomyces israelii, or Actinomyces bovis strains tested were susceptible. Results of restriction endonuclease analyses indicated that the genomes of these phages consisted of double-stranded DNA molecules ranging in size between 16 and 60 kbp. Sequence homology under hybridization conditions of high stringency was observed among a few of the isolated phages. A lysogenized isolate of A. viscosus MG-1 was obtained following infection with a temperate phage, designated phi 225. Results of Southern blot analyses indicated that phi 225 replicated as a plasmid in the lysogenized strain. Genomic DNA from several lytic phages was used to establish conditions for transfection by electroporation of strains of Actinomyces spp. Efficiencies of DNA transfer ranged from 10(2) to 10(5) plaque-forming units per microgram of DNA were obtained under optimal transfection conditions. The results of these studies demonstrate that transfer of genetic information in Actinomyces spp. can be achieved by transfection. (C) 1997 Academic Press. [References: 41]
机译：从放线菌的124个样品中的22个样品中分离出在放线菌菌株中产生混浊或澄清的裂解区的噬菌体。在这项研究中测试的所有人类和非人类的放线菌或纳氏放线菌菌株均对一种或多种这些噬菌体的感染敏感。相反，被测试的放线放线菌，以色列放线放线菌或牛放线放线菌均不敏感。限制性核酸内切酶分析的结果表明，这些噬菌体的基因组由大小在16至60 kbp之间的双链DNA分子组成。在少数分离的噬菌体中，在高度严格的杂交条件下观察到序列同源性。在用温带噬菌体感染后获得了经裂解的黏稠曲霉MG-1分离株，命名为phi225。Southern印迹分析的结果表明，phi 225作为质粒在溶菌菌株中复制。来自几种裂解噬菌体的基因组DNA被用于通过放线放线菌属菌株建立转染条件。在最佳转染条件下，每微克DNA可获得10（2）至10（5）噬菌斑形成单位的DNA转移效率。这些研究的结果表明，放线菌属中遗传信息的转移。可以通过转染来实现。 （C）1997学术出版社。 [参考：41]

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