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首页> 外文期刊>Plasmid: An International Journal Devoted to Extrachromosomal Gene Systems >THE JOINT OF TN916 CIRCULAR INTERMEDIATES IS A HOMODUPLEX IN ENTEROCOCCUS FAECALIS
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THE JOINT OF TN916 CIRCULAR INTERMEDIATES IS A HOMODUPLEX IN ENTEROCOCCUS FAECALIS

机译：TN916圆形中间体的连接是肠球菌粪便中的同形

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摘要

Tn916 is a 18-kb conjugative transposon originally identified in Enterococcus faecalis. The first step for Tn916 movement is its excision from the donor replicon with rha formation of a nonreplicating covalently closed circular intermediate. Studies on formation of circular intermediates in Escherichia coli have shown that the joint between the Tn916, termini is a 6-bp heteroduplex formed by the two regions flanking the transposon before its excision (coupling sequences), Ln this work we studied the joint of Tn916 termini in circular intermediates formed in both E. coli and E. faecalis. Our strategy was to use direct sequencing of amplification products obtained from the joint region of single target molecules. In E. coli, 50% of circular intermediates contained a heteroduplex joint, while the remaining 50% displayed a homoduplex joint formed by one of the hua coupling sequences, In E. faecalis, we could not demonstrate the presence of any heteroduplex joint. In this case 77.7% of the analyzed joints were formed by the left coupling sequence. (C) 1997 Academic Press. [References: 32]
机译：Tn916是最初在粪肠球菌中鉴定的18 kb结合转座子。 Tn916运动的第一步是将其从供体复制子中切除，形成非复制的共价封闭的圆形中间体。在大肠杆菌中形成环状中间体的研究表明，Tn916末端之间的接头是一个6 bp的异源双链体，由转座子被切除前两侧的两个区域（偶联序列）形成。在这项工作中，我们研究了Tn916的接头在大肠杆菌和粪肠球菌中形成的环状中间体中的末端。我们的策略是使用从单个靶分子联合区域获得的扩增产物的直接测序。在大肠杆菌中，50％的环状中间体含有一个异源双链接头，而其余的50％则显示了由hua偶联序列之一形成的同质双链接头。在粪肠球菌中，我们无法证明存在任何异源双链接头。在这种情况下，77.7％的分析关节是由左耦合顺序形成的。 （C）1997学术出版社。 [参考：32]

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