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首页> 外文期刊>Plasmid: An International Journal Devoted to Extrachromosomal Gene Systems >Comparative analyses of two cryptic plasmids from Haemophilus somnus (Histophilus somni)
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Comparative analyses of two cryptic plasmids from Haemophilus somnus (Histophilus somni)

机译：嗜血嗜血杆菌（Histophilus somni）的两个隐性质粒的比较分析

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Haemophihis somnus is an opportunistic bacterial pathogen capable of causing pneumonia, septicemia, and other systemic infections in bovines. An H. somnus isolate from bovine abortion (strain 649) was found to carry a similar to 1.3 kb plasmid (pHS649) that contained partial homology to two previously sequenced Haemophilus/Histophilus plasmids by BLAST analyses. Sequence analysis of pHS649 identified a putative RepA protein with 48% similarity to the RepA protein of Escherichia coli plasmid pKL1. A similar to 5 kb plasmid (pHs129) from H. somnus preputial isolate 129Pt was also sequenced and found to encode two copies of a putative RepB protein. Whereas pHS649 stably replicated in E coli DH5 alpha, pHS129 did not. Genetic relatedness and possible replication mechanisms of these plasmids are described. Published by Elsevier Inc.
机译：嗜血嗜血杆菌是一种机会细菌病原体，能够引起牛的肺炎，败血病和其他全身性感染。发现来自牛流产的猪链球菌分离株（菌株649）带有一个与1.3 kb质粒（pHS649）相似的质粒，该质粒通过BLAST分析与两个先前测序的嗜血杆菌/嗜血杆菌质粒具有部分同源性。 pHS649的序列分析确定了一种推定的RepA蛋白，与大肠杆菌质粒pKL1的RepA蛋白具有48％的相似性。还对来自嗜血幽门螺杆菌分离株129Pt的5kb质粒（pHs129）进行了测序，发现其编码两个拷贝的推定RepB蛋白。 pHS649可在大肠杆菌DH5 alpha中稳定复制，而pHS129则不能。描述了这些质粒的遗传相关性和可能的​​复制机制。由Elsevier Inc.发布

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