Construction of a highly-active, liver-specific transcriptional regulatory element through combination of the albumin promoter and alpha-fetoprotein enhancer.

Chen EQ; Song XQ; Wang YL; Zhou TY; Bai L; Liu L; Liu C; Cheng X; Tang H

首页> 外文期刊>Plasmid: An International Journal Devoted to Extrachromosomal Gene Systems >Construction of a highly-active, liver-specific transcriptional regulatory element through combination of the albumin promoter and alpha-fetoprotein enhancer.

【24h】

Construction of a highly-active, liver-specific transcriptional regulatory element through combination of the albumin promoter and alpha-fetoprotein enhancer.

机译：通过结合白蛋白启动子和甲胎蛋白增强子，构建高活性，肝特异性转录调控元件。

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

In an attempt to construct a highly active, liver-specific transcriptional regulatory element, the mouse albumin promoter (ALBp) and alpha-fetoprotein enhancer (AFPe) were obtained. To verify its hepatic specificity and activity, the AFPe-ALBp-containing fragment was cloned into the plasmids, pVAX-S and pGL3-Luc with original promoter removed. Plasmid pVAX-AFPe-ALBp-S was then transfected into hepatic and non-hepatic cells in vitro, and delivered into mouse by intravenous injection and intramuscular injection, respectively. In addition, pGL3-AFPe-ALBp-Luc was transfected into hepatic and non-hepatic cell lines; pVAX1, pVAX1/S, and pGL3-ALBp-Luc were used as controls. The expression of hepatitis B surface antigen (HBsAg) was observed, and luciferase activity in cells was measured. For plasmid pVAX-AFPe-ALBp-S, the expression of HBsAg was observed in hepatic cell lines, but not in a non-hepatic cell line. Using pVAX-S, the expression of HBsAg was observed in both hepatic and non-hepatic cell lines. In cells expressing pGL3-AFPe-ALBp-Luc, the level of luciferase activity was significantly higher in hepatic cell lines, compared with the non-hepatic cell lines. In addition, the level of luciferase activity in cells expressing pGL3-AFPe-ALBp-Luc was significantly higher than that of pGL3-ALBp-Luc in hepatic cell lines, suggesting that AFPe could enhance target gene expression under the control of ALBp. The expression of HBsAg was detected in mouse liver, but not muscle when using pVAX-AFPe-ALBp-S. In contrast, the expression of HBsAg was detected in both mouse liver and muscle upon transfection with pVAX-S. In conclusion, the AFPe-ALBp element could be used as a tool to induce liver-specific expression of a target gene.

机译：为了构建高活性，肝特异性转录调节元件，获得了小鼠白蛋白启动子（ALBp）和甲胎蛋白增强子（AFPe）。为了验证其肝特异性和活性，将含有AFPe-ALBp的片段克隆到质粒pVAX-S和pGL3-Luc中，并去除了原始启动子。然后将质粒pVAX-AFPe-ALBp-S体外转染到肝细胞和非肝细胞中，并分别通过静脉内注射和肌肉内注射递送到小鼠中。另外，将pGL3-AFPe-ALBp-Luc转染到肝和非肝细胞系中。将pVAX1，pVAX1 / S和pGL3-ALBp-Luc用作对照。观察乙型肝炎表面抗原（HBsAg）的表达，并测量细胞中的萤光素酶活性。对于质粒pVAX-AFPe-ALBp-S，在肝细胞系中观察到HBsAg的表达，但在非肝细胞系中未观察到。使用pVAX-S，在肝细胞和非肝细胞系中均观察到HBsAg的表达。在表达pGL3-AFPe-ALBp-Luc的细胞中，与非肝细胞系相比，肝细胞系中的荧光素酶活性水平明显更高。另外，在肝细胞系中表达pGL3-AFPe-ALBp-Luc的细胞中荧光素酶活性水平明显高于pGL3-ALBp-Luc，这表明AFPe可以在ALBp的控制下增强靶基因表达。使用pVAX-AFPe-ALBp-S时，在小鼠肝脏中检测到HBsAg表达，但未检测到肌肉。相反，用pVAX-S转染后，在小鼠肝脏和肌肉中均检测到HBsAg的表达。总之，AFPe-ALBp元件可用作诱导靶基因肝脏特异性表达的工具。

著录项

来源
《Plasmid: An International Journal Devoted to Extrachromosomal Gene Systems》 |2011年第2期|共7页
作者
Chen EQ; Song XQ; Wang YL; Zhou TY; Bai L; Liu L; Liu C; Cheng X; Tang H;
展开▼
作者单位

展开▼
收录信息
原文格式 PDF
正文语种 eng
中图分类医学遗传学;
关键词

相似文献

外文文献
中文文献
专利

1. Construction of a highly-active, liver-specific transcriptional regulatory element through combination of the albumin promoter and alpha-fetoprotein enhancer. [J] . Chen EQ, Song XQ, Wang YL, Plasmid: An International Journal Devoted to Extrachromosomal Gene Systems . 2011,第2期

机译：通过结合白蛋白启动子和甲胎蛋白增强子，构建高活性，肝特异性转录调控元件。
2. Cellular promoters incorporated into the adenovirus genome: effects of viral regulatory elements on transcription rates and cell specificity of albumin and beta-globin promoters. [J] . L E Babiss, J M Friedman, J E Darnell Molecular and Cellular Biology . 1986,第11期

机译：掺入腺病毒基因组中的细胞促进剂：病毒调节元件对白蛋白和β-珠蛋白启动子的转录速率和细胞特异性的影响。
3. Cellular promoters incorporated into the adenovirus genome: effects of viral regulatory elements on transcription rates and cell specificity of albumin and beta-globin promoters. [J] . L E Babiss, J M Friedman, J E Darnell Molecular and Cellular Biology . 1986,第11期

机译：掺入腺病毒基因组中的细胞促进剂：病毒调节元件对白蛋白和β-珠蛋白启动子的转录速率和细胞特异性的影响。
4. Distance conservation of transcription regulatory motifs in saccharomyces cerevisiae promoters [C] . Qin Dandan, Lu Jun, Ding Changjiang, 2010 3rd International Conference on Biomedical Engineering and Informatics . 2010

机译：酿酒酵母启动子中转录调控基序的距离保守
5. Therapeutic Effect of Adenovirus- and alpha-fetoprotein Promoter-mediated tBid and Chemotherapeutic Agents in Combination on Orthotopic Hepatocellular Carcinoma in Mice. [D] . Ma, Shihong. 2010

机译：腺病毒和甲胎蛋白启动子介导的tBid和化学治疗剂联合治疗对小鼠原位肝细胞癌的治疗作用。
6. Cellular promoters incorporated into the adenovirus genome: effects of viral regulatory elements on transcription rates and cell specificity of albumin and beta-globin promoters. [O] . L E Babiss, J M Friedman, J E Darnell Jr 1986

机译：纳入腺病毒基因组的细胞启动子：病毒调节元件对白蛋白和β-珠蛋白启动子的转录速率和细胞特异性的影响。
7. Cellular promoters incorporated into the adenovirus genome: effects of viral regulatory elements on transcription rates and cell specificity of albumin and beta-globin promoters. [O] . Babiss, L E, Friedman, J M, Darnell, J E 1986

机译：纳入腺病毒基因组的细胞启动子：病毒调节元件对白蛋白和β-珠蛋白启动子的转录速率和细胞特异性的影响。

Construction of a highly-active, liver-specific transcriptional regulatory element through combination of the albumin promoter and alpha-fetoprotein enhancer.

摘要

著录项

相似文献

相关主题

期刊订阅