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首页> 外文期刊>Plasmid: An International Journal Devoted to Extrachromosomal Gene Systems >Analysis of the requirement for a pUB110 mob region during Tn916-dependent mobilization.
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Analysis of the requirement for a pUB110 mob region during Tn916-dependent mobilization.

机译：Tn916依赖动员期间对pUB110暴民区的需求分析。

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摘要

Tn916-dependent mobilization of nonconjugative plasmids pUB110 and its derivative pUB110Deltam was compared. Deleting a 787-bp fragment from the pUB110 mob region created plasmid pUB110Deltam. Deletion of the mob region of pUB110 rendered the plasmid nontransferable by the conjugative plasmids of Bacillus thuringiensis subsp. israelensis. During matings between Bacillus subtilis (Tn916) and B. thuringiensis subsp. israelensis, however, Tn916-dependent mobilization of plasmids pUB110 and pUB110Deltam was observed at a frequency of approximately 2 x 10(-6) transconjugants per donor. The results show that Tn916-mediated conjugal transfer of plasmids is a mob-independent event. Jaworski and Clewell (J. Bacteriol 177; 6644-6651) recently demonstrated the presence of an IncP-like nicking site in the oriT of Tn916. These data suggest that a IncP-like nickling site is essential for Tn916-mediated plasmid transfer. Copyright 1999 Academic Press.
机译：比较了非结合质粒pUB110及其衍生物pUB110Deltam的Tn916依赖动员。从pUB110 mob区域中删除一个787bp的片段，产生了质粒pUB110Deltam。 pUB110的mob区的缺失使得该质粒不能被苏云金芽孢杆菌亚种的结合质粒转移。以色列。在枯草芽孢杆菌（Tn916）和苏云金芽孢杆菌亚种交配期间。但是，在每个供体中，观察到质粒pUB110和pUB110Deltam的Tn916依赖动员的频率约为2 x 10（-6）个转导结合体。结果表明，Tn916介导的质粒的夫妻转移是不依赖暴民的事件。 Jaworski和Clewell（J. Bacteriol 177; 6644-6651）最近证明了在Tn916的原位中存在类似IncP的切刻位点。这些数据表明，IncP样的切口位点对于Tn916介导的质粒转移至关重要。版权所有1999 Academic Press。

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