Identification of a potential membrane-targeting region of the replication initiator protein (TrfA) of broad-host-range plasmid RK2.

Kim PD; Rosche TM; Firshein W

首页> 外文期刊>Plasmid: An International Journal Devoted to Extrachromosomal Gene Systems >Identification of a potential membrane-targeting region of the replication initiator protein (TrfA) of broad-host-range plasmid RK2.

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Identification of a potential membrane-targeting region of the replication initiator protein (TrfA) of broad-host-range plasmid RK2.

机译：鉴定宽宿主范围质粒RK2的复制起始蛋白（TrfA）的潜在膜靶向区域。

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Plasmid RK2 codes for two species of the replication initiator protein TrfA (33 and 44 kDa). Both polypeptides are strongly associated with membrane fractions of Escherichia coli host cells (W. Firshein and P. Kim, Mol. Microbiol. 23, 1-10, 1997). We investigated the role of a 12-amino-acid hydrophobic region (HR) in the membrane association of TrfA. Epitope-tagged polypeptide fragments of TrfA that contained HR were expressed and found to be associated with membrane fractions. Site-directed mutagenesis of trfA revealed that changes of specific amino acids in HR can affect both TrfA association with the membrane and its ability to support replication of an RK2 oriV plasmid in vivo. These results are consistent with the hypothesis that membrane association of TrfA is functionally relevant and that the HR region of TrfA is involved in membrane association and DNA replication in vivo. Copyright 2000 Academic Press.

机译：质粒RK2编码两种复制起始蛋白TrfA（33和44 kDa）。两种多肽均与大肠杆菌宿主细胞的膜级分密切相关（W.Firshein和P.Kim，分子微生物学，23，1-10，1997）。我们调查了TrfA的膜缔合中的12个氨基酸疏水区（HR）的作用。表达含有HR的TrfA的表位标记多肽片段，发现与膜级分相关。 trfA的定点诱变表明，HR中特定氨基酸的变化会影响TrfA与膜的结合及其在体内支持RK2 oriV质粒复制的能力。这些结果与TrfA的膜缔合在功能上相关并且TrfA的HR区参与体内的膜缔合和DNA复制的假设相一致。版权所有2000学术出版社。

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《Plasmid: An International Journal Devoted to Extrachromosomal Gene Systems》 |2000年第3期|共9页
作者
Kim PD; Rosche TM; Firshein W;
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正文语种 eng
中图分类医学遗传学;
关键词
Bacterial Proteins; Cell Membrane; Plasmids; Peptide Fragments; TrfA protein; 细菌蛋白质类; 细胞膜; 质粒;

机译：Bacterial Proteins;Cell Membrane;Plasmids;Peptide Fragments;TrfA protein;细菌蛋白质类;细胞膜;质粒;

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1. Identification of a potential membrane-targeting region of the replication initiator protein (TrfA) of broad-host-range plasmid RK2. [J] . Kim PD, Rosche TM, Firshein W Plasmid: An International Journal Devoted to Extrachromosomal Gene Systems . 2000,第3期

机译：鉴定宽宿主范围质粒RK2的复制起始蛋白（TrfA）的潜在膜靶向区域。
2. Analysis of mutations in trfA, the replication initiation gene of the broad-host-range plasmid RK2. [J] . J Lin, D R Helinski Journal of bacteriology . 1992,第12期

机译：trfA突变的分析，trfA是宽宿主范围质粒RK2的复制起始基因。
3. Replication of the broad-host-range plasmid RK2: direct measurement of intracellular concentrations of the essential TrfA replication proteins and their effect on plasmid copy number. [J] . R H Durland, D R Helinski Journal of bacteriology . 1990,第7期

机译：广泛宿主质粒RK2的复制：直接测量必需TrfA复制蛋白的细胞内浓度及其对质粒拷贝数的影响。
4. Inhibition of Epithelial Tumor Cell Replication by the alpha3 chain of Type IV Collagen Is Initiated Through Its Interaction with Integrin Associated Protein (CD47) and Integrin beta3 [C] . T.A. Shahan, S. Pasco, A. Fawzi, NATO Advanced Study Institute on Vascular Endothelium : Mechanisms of Cell Signaling . 1999

机译：通过与整联蛋白相关蛋白（CD47）的相互作用引发IV型胶原醛基链的上皮肿瘤细胞复制的抑制通过其与整合蛋白β3引发
5. Pheromone-interacting replication protein controls enterococcal conjugative plasmid host range and stability through disulfide bonds. [D] . Utter, Bryan. 2012

机译：信息素相互作用复制蛋白通过二硫键控制肠球菌结合质粒的宿主范围和稳定性。
6. Analysis of mutations in trfA the replication initiation gene of the broad-host-range plasmid RK2. [O] . J Lin, D R Helinski 1992

机译：trfA突变的分析trfA是宽宿主范围质粒RK2的复制起始基因。
7. Analysis of mutations in trfA, the replication initiation gene of the broad-host-range plasmid RK2. [O] . Lin, J, Helinski, D R 1992

机译：trfA突变的分析，trfA是宽宿主范围质粒RK2的复制起始基因。
8. DNA Replication Initiator Proteins and Genetic Instability: Creating a Mouse Model for Prostate Cancer [R] . Jackson, P. K. 2000

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Identification of a potential membrane-targeting region of the replication initiator protein (TrfA) of broad-host-range plasmid RK2.

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