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首页> 外文期刊>Plasmid: An International Journal Devoted to Extrachromosomal Gene Systems >The novel insertion sequences IS1417, IS1418, and IS1419 from Burkholderia glumae and their strain distribution.
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The novel insertion sequences IS1417, IS1418, and IS1419 from Burkholderia glumae and their strain distribution.

机译：伯克霍尔德氏菌的新插入序列IS1417，IS1418和IS1419及其菌株分布。

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摘要

Three insertion sequences, IS1417, IS1418, and IS1419, were isolated from Burkholderia glumae (formerly Pseudomonas glumae), a gram-negative rice pathogenic bacterium, on the basis of their abilities to activate the expression of the neo gene of the entrap vector pSHI1063. The 1335-bp IS1417 element with 17-bp imperfect terminal inverted repeats was found to be flanked by 5-bp direct repeats of the vector sequence. IS1418 is 865 bp in length and carries 15-bp inverted repeats with a target duplication of 3 bp. The 1215-bp IS1419 sequence is bounded by the 36-bp terminal inverted repeats of the element and 7-bp direct repeats of the vector sequence. IS1417 and IS1418 belong to the IS2 subgroup of the IS3 family and the IS427 subgroup of the IS5 family, respectively, whereas IS1419 does not appear to be a member of any known IS family. Southern blot analysis of DNAs from B. glumae field isolates indicated that those IS elements are widely distributed, but the host range of the three IS elements appears to be limited to B. glumae and some other related species such as B. plantarii. The polymorphisms exhibited in B. glumae isolates suggest that those elements are useful for molecular epidemiological studies of B. glumae infections. Copyright 2000 Academic Press.
机译：基于它们能激活包埋载体pSHI1063的neo基因表达的能力，从革兰氏阴性致病菌伯克霍尔德氏菌（原名假单胞菌）分离了三个插入序列IS1417，IS1418和IS1419。发现具有17 bp不完美末端反向重复序列的1335 bp IS1417元件侧翼是载体序列的5 bp直接重复序列。 IS1418长度为865 bp，带有15 bp的反向重复序列，目标重复序列为3 bp。 1215-bp IS1419序列由元件的36-bp末端反向重复序列和载体序列的7-bp直接重复序列限制。 IS1417和IS1418分别属于IS3家族的IS2子组和IS5家族的IS427子组，而IS1419似乎不是任何已知IS家族的成员。对来自B. glumae野外分离株的DNA的Southern印迹分析表明，这些IS元件分布广泛，但三个IS元件的宿主范围似乎仅限于B. glumae和一些其他相关物种，例如植物双歧杆菌。在小芽孢杆菌中分离出的多态性表明，这些元素可用于小芽孢杆菌感染的分子流行病学研究。版权所有2000学术出版社。

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