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首页> 外文期刊>Plasmid: An International Journal Devoted to Extrachromosomal Gene Systems >In vitro analysis of the terminator T(II) of the inhibitor antisense rna II gene from plasmid pMV158.
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In vitro analysis of the terminator T(II) of the inhibitor antisense rna II gene from plasmid pMV158.

机译:质粒pMV158的抑制剂反义rna II基因终止子T(II)的体外分析。

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摘要

One element involved in the copy number control of plasmid pMV158 is the antisense RNA II. We have determined the precise size of this RNA synthesized in vitro. Transcript termination occurs at two consecutive template positions within a typical rho-independent terminator, T(II). Using a series of plasmid derivatives in which the T(II) terminator has been partially or totally removed, we have analyzed the relationship between the predicted stability of the RNA hairpin encoded by T(II) and the efficiency of in vitro intrinsic termination. All the plasmids of the pMV158 family, constituted so far of 19 replicons, harbor putative antisense RNA-encoding genes which share in common the relative location with respect to the essential rep gene, the small size, and the presence of rho-independent transcription terminators. Copyright 2001 Academic Press.
机译:与质粒pMV158的拷贝数控制有关的一个元件是反义RNA II。我们已经确定了该RNA在体外合成的精确大小。转录终止发生在典型的rho独立终止子T(II)中的两个连续的模板位置。使用一系列的质粒衍生物,其中T(II)终止子已被部分或全部去除,我们分析了由T(II)编码的RNA发夹的预测稳定性与体外内在终止效率之间的关系。迄今为止,pMV158家族的所有质粒均由19个复制子组成,带有推定的反义RNA编码基因,它们相对于必需rep基因具有相对位置,较小的大小以及不依赖rho的转录终止子。版权所有2001,学术出版社。

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