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首页> 外文期刊>Preparative biochemistry & biotechnology: An international journal for rapid communication >Electrophoretic separation of gill proteins of the clam Codakia orbicularis
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Electrophoretic separation of gill proteins of the clam Codakia orbicularis

机译：电泳分离圆纹蛤g蛋白质

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Marine organisms inhabiting the coastal environment of the Caribbean islands have attracted the attention of a number of,research scientists. These organisms generally live in areas of high environmental stress and may, therefore, contain specialized proteins and enzymes which exhibit valuable biotechnological applications. Among them, a small number of clams have been studied. Our work relates to the clam Codakia orbicularis. This bivalve lives in areas of high hydrogen sulfide concentrations on the Caribbean island of Guadeloupe. Its enzymatic system must, therefore, have evolved to allow its adaptation to this high-stress environment. C. orbicularis also contains endosymbiotic bacteria which are housed in the bacteriocytes of the gills. Its protein content can, therefore, be expected to have an impact on this symbiotic relationship. We have analysed gill protein extracts of this clam by various biochemical techniques: SDS-PAGE, IEF, PAS, and Western blotting using a panel of lectins, in order to establish its protein and glycoprotein profiles. This biochemical analysis, the first of its kind, constitutes an important step in separating and characterizing the proteins involved in the biochemical pathways of this organism whose stock is in decline in Guadeloupe. Our results show the presence of three major proteins whose molecular weights vary between 14,000 and 24,000 Daltons, and some of which are glycoproteins with predominantly oc-mannose and N-acetylgalactosamine moieties. Their pI values are in the range between 4.5 and 5.6. These protein profiles are different from those observed for Lucina pectinata, a clam which has been the subject of earlier studies in the literature. [References: 12]
机译：居住在加勒比海岛屿沿海环境中的海洋生物引起了许多研究科学家的关注。这些生物通常生活在高环境压力的地区，因此可能含有特殊的蛋白质和酶，这些蛋白质和酶具有重要的生物技术应用价值。其中，已经研究了少数蛤lam。我们的工作与蛤Co Codakia orbicularis有关。这种双壳类动物生活在加勒比海瓜德罗普岛上硫化氢浓度高的地区。因此，它的酶系统必须已经进化以使其适应这种高压力环境。轮状梭菌还含有内共生细菌，它们位于the的细菌细胞中。因此，可以预期它的蛋白质含量会影响这种共生关系。我们已经通过各种生化技术分析了这种蛤g蛋白质的提取物：SDS-PAGE，IEF，PAS和使用一组凝集素的蛋白质印迹，以建立其蛋白质和糖蛋白谱。这种生化分析尚属首次，它是分离和表征该生物体生化途径中涉及的蛋白质的重要​​步骤，该生物体的存量在瓜德罗普岛呈下降趋势。我们的结果表明存在三种主要蛋白质，其分子量在14,000至24,000道尔顿之间变化，其中一些是主要具有oc-甘露糖和N-乙酰半乳糖胺部分的糖蛋白。它们的pI值在4.5到5.6之间。这些蛋白质谱不同于已观察到的蛤LucLucina pectinata的蛋白谱。 [参考：12]

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