Comparative Study of Three Methods for Non-radioactive In Vivo DNA Labeling in Escherichia coli Using Nucleoside Analogs

Dannelys Perez-Bello; Viana Manrique Suarez; Celso Perez Bolanos; Judith Miranda Lopez; Gabriel Perez Perez; Lilia Lopez-Canovas; David Higginson-Clarke; Chryslaine Rodriguez-Tanty; Ana Maria Riveron

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Comparative Study of Three Methods for Non-radioactive In Vivo DNA Labeling in Escherichia coli Using Nucleoside Analogs

机译：使用核苷类似物在大肠杆菌中进行非放射性体内DNA标记的三种方法的比较研究

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In the present work,a comaprative study of 5-FdUrd,thy~-,and metabolic in vivo labeling methods for plasmid and chromosomal DNA in E.coli DH5alpha cells was performed in order to achieve the best thymidine substitution method by 5-BrdUrd.According to the colorimetric immunoenzymatic results,we found that the minimal detectable labeled DNA (MDLD) was 312pg with the 5-FdUrd and thy~- methods for 5-BrdUrd labeled plasmid DNA.5-BrdUrd replaced about 96% of the total thymidine by 5-FdUrd methods;for the thy~- and metabolic labeling methods,the MDLD value was 1,25 ng for denatured 5-BrdUrd chromosomal DNA.Pyrimidine nucleoside analogues were also evaluated as immunochemical markers for their in vivo introduction into DNA.

机译：在本工作中，对大肠杆菌DH5alpha细胞中质粒和染色体DNA的5-FdUrd，胸腺和代谢体内标记方法进行了协同研究，以实现5-BrdUrd最佳的胸苷替代方法。根据比色免疫酶法的结果，我们发现5-FdUrd和thy〜-方法对5-BrdUrd标记的质粒DNA的最小可检测标记DNA（MDLD）为312pg。5-BrdUrd替代了约96％的胸苷5-FdUrd方法;对于thy-和代谢标记方法，变性的5-BrdUrd染色体DNA的MDLD值为1.25 ng。嘧啶核苷类似物也被用作免疫化学标记物，用于将其体内引入DNA。

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《Preparative biochemistry & biotechnology: An international journal for rapid communication》 |2003年第4期|共16页
作者
Dannelys Perez-Bello; Viana Manrique Suarez; Celso Perez Bolanos; Judith Miranda Lopez; Gabriel Perez Perez; Lilia Lopez-Canovas; David Higginson-Clarke; Chryslaine Rodriguez-Tanty; Ana Maria Riveron;
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正文语种 eng
中图分类生物化学;
关键词
5-BrdUrd; non-radioactive probe; in vivo labeling methods; pyrimidine nucleoside analoguesy;

机译：5-BrdUrd;非放射性探针;体内标记方法;嘧啶核苷类似物;

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1. Comparative Study of Three Methods for Non-radioactive In Vivo DNA Labeling in Escherichia coli Using Nucleoside Analogs [J] . Dannelys Perez-Bello, Viana Manrique Suarez, Celso Perez Bolanos, Preparative biochemistry & biotechnology: An international journal for rapid communication . 2003,第4期

机译：使用核苷类似物在大肠杆菌中进行非放射性体内DNA标记的三种方法的比较研究
2. A thymidineless Escherichia coli strain useful for in vivo DNA labeling [J] . Ana María Riverón Rojas, Celso Pérez Bola?os, Chryslaine Rodríguez Tanty, Revista CENIC Ciencias Biologicas . 2004,第2期

机译：一种无胸苷的大肠杆菌菌株，可用于体内DNA标记
3. Location of protein S1 of Escherichia coli ribosomes at the ‘A'-site of the codon binding site. Affinity labeling studies with a 3′ -modified A-U-G analog [J] . Georg St?ffler, Olaf Pongs, Rolf W. Bald Nucleic acids research . 1976,第7期

机译：大肠杆菌核糖体蛋白S1在密码子结合位点“ A”位的位置。用3'修饰的A-U-G类似物进行亲和标记研究
4. Substantial Increasement of 2,6-Diaminopurine Nucleosides Production by Co-Expression of Recombinant Nucleoside Phosphorylase from Escherichia Coli K12 [C] . The 12th Asian Pacific Confederation of Chemical Engineering Congress(第十二届亚太化工联盟大会暨化工展览会)论文集 . 2008

机译：通过共表达大肠杆菌K12的重组核苷磷酸化酶共表达，大大增加了2,6-二氨基嘌呤核苷的生产
5. Experimental and computational studies of the in vivo genomic repertoire of DNA -protein interactions in Escherichia coli. [D] . Vora, Tiffany Jayshree. 2007

机译：大肠杆菌中DNA-蛋白质相互作用的体内基因组库的实验和计算研究。
6. Mutagenic nucleoside analog N4-aminocytidine: metabolism incorporation into DNA and mutagenesis in Escherichia coli. [O] . K Negishi, K Tamanoi, M Ishii, 1988

机译：诱变的核苷类似物N4-氨基胞嘧啶核苷：在大肠杆菌中代谢掺入DNA和诱变。
7. Mutagenic nucleoside analog N4-aminocytidine: metabolism, incorporation into DNA, and mutagenesis in Escherichia coli. [O] . Negishi, K, Tamanoi, K, Ishii, M, 1988

机译：诱变的核苷类似物N4-氨基胞嘧啶核苷：在大肠杆菌中代谢，掺入DNA和诱变。

Comparative Study of Three Methods for Non-radioactive In Vivo DNA Labeling in Escherichia coli Using Nucleoside Analogs

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