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Use of natural dye-casein complexes: Effect of proteolytic treatment

机译:天然染料-酪蛋白配合物的使用:蛋白水解处理的效果

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摘要

The activity of proteolytic enzymes is commonly measured using casein as a substrate. A modified caseinolysis assay was developed with natural dyes such as juglone, lawsone, berberine, and quercetin for Subtilisin carlsberg , protease type XVI, and trypsin, respectively. The pH dependence and incubation time were determined. K-m, V-max, and k(cat)/K-m values were also determined for these enzymes. Lawsone was found to be a better substrate than the others.
机译:通常使用酪蛋白作为底物来测量蛋白水解酶的活性。针对枯草杆菌蛋白酶嘉士伯,蛋白酶XVI和胰蛋白酶的天然染料,如juglone,lawsone,小ber碱和槲皮素,开发了一种改良的酪蛋白水解测定法。确定pH依赖性和温育时间。还确定了这些酶的K-m,V-max和k(cat)/ K-m值。劳森被发现是比其他更好的基质。

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