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首页> 外文期刊>Preparative biochemistry & biotechnology: An international journal for rapid communication >NAD~+ Mediated Differential Thermotolerance Between Chloroplastic and Cytosolic L-myo-Inositol-1-phosphate Synthase from Diptopterygium glaucum (Thunb.) Nakai
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NAD~+ Mediated Differential Thermotolerance Between Chloroplastic and Cytosolic L-myo-Inositol-1-phosphate Synthase from Diptopterygium glaucum (Thunb.) Nakai

机译：NAD〜+介导的青枯对叶拟南芥的叶绿体和胞质L-肌醇1磷酸合酶之间的差异热耐受性

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摘要

Relative thermotolerance of the enzyme,L-myo-inositol-1-phosphate synthase (MIPS; EC: 5.5.1.4),from the chloroplastic and cytosolic sources of Diptopterygium glaucum was studied.The purification involved streptomycin sulphate precipitation,ammonium sulphate fractionation,ion-exchange chromatography,and molecular sieve chromatography.After the final chromatography,16.62% of chloroplastic and 13.47% of cytosolic MIPS could be recovered.Between 15 deg C and 55 deg C,the two forms of MIPS exhibited differential thermal stability,which is related to the presence of the MIPS co-factor,NAD~+.Added NAD~+ increased the lower thermotolerance of the chloroplastic MIPS and the removal of 'built-in' NAD~+ decreased the higher thermal stability of the cytosolic MIPS.
机译：研究了来自双翅目青霉的叶绿体和胞质来源的L-肌醇-1-磷酸合酶（MIPS; EC：5.5.1.4）的相对耐热性。纯化过程包括硫酸链霉素沉淀，硫酸铵分级分离，离子交换色谱和分子筛色谱。最终色谱后，可回收到16.62％的叶绿体和13.47％的胞质MIPS。在15℃至55℃之间，两种形式的MIPS表现出不同的热稳定性，这与添加NAD〜+会增加叶绿体MIPS的较低耐热性，而去除“内置” NAD〜+则会降低胞质MIPS的较高热稳定性。

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