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首页> 外文期刊>Peptides: An International Journal >Aplysia seductin is a water-borne protein pheromone that acts in concert with attractin to stimulate mate attraction.
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Aplysia seductin is a water-borne protein pheromone that acts in concert with attractin to stimulate mate attraction.

机译：Aplysia诱惑是一种水性蛋白质信息素，可与引力协同作用以刺激伴侣的引力。

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Mate attraction in Aplysia involves the long-distance water-borne protein pheromones attractin, enticin, and temptin which are released during egg-laying. Other water-borne pheromones are predicted to act in concert with attractin, enticin, and temptin, but their identities were unknown. We recently identified a highly expressed Aplysia californica albumen gland gene (Alb-23) that encoded a novel protein by differential library screening of an albumen gland cDNA library. To determine whether Alb-23 ('seductin') was a water-borne pheromone, we employed Western blot analysis, purification and expression of albumen gland proteins, immunolocalization studies, pheromone secretion assays, comparative genomics, and behavioral bioassays. Immunoreactive seductin was detected in eluates of egg cordons, indicating that seductin was secreted onto the cordon during egg laying. Aplysia brasiliana seductin was 94% identical to its A.californica homolog. In T-maze attraction assays, the combination of attractin and seductin was significantly attractive to potential mates, whereas either protein alone was not. Data from this and previous studies support the hypothesis that seductin is a water-borne protein pheromone that acts in concert with attractin, enticin, and temptin to attract Aplysia to form and maintain mating aggregations.
机译：在海ly中的伴侣吸引作用涉及长距离的水性蛋白信息素引诱素，enticin和temptin，它们在产卵过程中释放。预计其他水性信息素与引诱剂，enticin和temptin协同作用，但它们的身份尚不清楚。我们最近确定了一个高表达的海百合白蛋白腺基因（Alb-23），该蛋白通过对蛋白腺cDNA文库的差异文库筛选来编码一种新蛋白质。为了确定Alb-23（“ seductin”）是否为水性信息素，我们采用蛋白质印迹分析，蛋白和腺蛋白的纯化与表达，免疫定位研究，信息素分泌测定，比较基因组学和行为生物测定。在卵子警戒线的洗脱液中检测到了免疫反应性的seductin，这表明在产卵过程中seductin分泌到警戒线上。巴西海螺Seductin与它的加利福尼亚农杆菌同系物94％相同。在T迷宫吸引试验中，引诱素和诱集素的组合对潜在配偶具有明显的吸引力，而单独的任一蛋白都不具有吸引力。来自本研究和先前研究的数据支持以下假设：seductin是一种水性蛋白质信息素，可与吸引素，enticin和temptin协同作用，以吸引Aplysia形成并维持交配聚集。

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