...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Biomaterials Science >Ambient temperature synthesis of citrate stabilized and biofunctionalized, fluorescent calcium fluoride nanocrystals for targeted labeling of cancer cells
【24h】

Ambient temperature synthesis of citrate stabilized and biofunctionalized, fluorescent calcium fluoride nanocrystals for targeted labeling of cancer cells

机译：柠檬酸盐稳定和生物功能化的荧光氟化钙纳米晶体的环境温度合成，用于癌细胞的靶向标记

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Targeted biological contrast agents are emerging as promising candidates in the field of cancer theragnostics. Herein, we report an ambient temperature synthesis of a nanosized, antibody functionalized lanthanide doped CaF_2 biolabel and demonstrate in vitro its potential for cancer cell targeting efficacy and specificity. Monodispersed citrate stabilized lanthanide (Eu~(3+)) doped CaF_2 nanoparticles with size ～25 nm, exhibiting strong fluorescent emission at 612 nm, were prepared using an aqueous wet chemical route at room temperature. Biofunctionalization of the fluorescent nanoparticles using an anti-EGFR antibody through EDC-NHS coupling chemistry enabled targeting of EGFR over-expressing cells. The nanobioconjugates showed preferential binding to EGFR~(+ve) oral epithelial carcinoma cells (KB) and human epidermoid carcinoma cells (A431) with no accumulation onto EGFR~(-ve) non-cancerous NIH 3T3 cells. The fluorescence was maintained after the bioconjugation as well as after attachment to the cancer cells, demonstrating their potential as targeted biolabels. Cytotoxicity evaluation with several cancerous (A431, KB) and non-cancerous (NIH 3T3, L929) cell lines revealed no toxicity at concentrations up to 1 mM. Thus, the fluorescence characteristics and biocompatibility, coupled with the molecular receptor targeting capability, suggest the potential use of CaF_2 in the field of bioimaging.
机译：在癌症治疗学领域中，靶向生物造影剂正在成为有希望的候选者。在这里，我们报告了纳米级，抗体功能化镧系元素掺杂的CaF_2生物标记物的环境温度合成，并在体外证明其潜在的癌细胞靶向功效和特异性。采用室温湿化学法制备了单分散的柠檬酸盐稳定的镧系元素（Eu〜（3+））掺杂的CaF_2纳米粒子，尺寸约为〜25 nm，在612 nm处具有较强的荧光发射。使用抗EGFR抗体通过EDC-NHS偶联化学技术对荧光纳米粒子进行生物功能化，可以靶向过表达EGFR的细胞。纳米生物共轭物表现出与EGFR〜（+ ve）口腔上皮癌细胞（KB）和人表皮样癌细胞（A431）的优先结合，而没有聚集在EGFR〜（-ve）非癌NIH 3T3细胞上。生物缀合后以及附着于癌细胞后，荧光得以保持，证明了其作为靶向生物标记物的潜力。对几种癌性（A431，KB）和非癌性（NIH 3T3，L929）细胞系的细胞毒性评估显示，浓度高达1 mM时无毒性。因此，荧光特性和生物相容性，再加上分子受体靶向能力，表明CaF_2在生物成像领域的潜在用途。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号