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首页> 外文期刊>Biomaterials Science >Immobilization of Sertoli cells on islets of Langerhans
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Immobilization of Sertoli cells on islets of Langerhans

机译：Sertoli细胞在Langerhans胰岛上的固定化

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Sertoli cells play a crucial role in creating the immunoprivileged environment of the testis. We examined the survival of islets of Langerhans after co-transplantation with Sertoli cells. Sertoli cells near islets should protect the graft from rejection. In this study, conjugates of single stranded oligonucleotides, poly(ethylene glycol) and phospholipids (ssDNA-PEG-DPPE) were used to immobilize Sertoli cells on islets. The 20-mer of deoxyadenylic acid (oligo(dA)_(20)) and 20-mer of deoxythymidylic acid (oligo(dT)_(20)) were presented as ssDNAs on the surfaces of Sertoli cells and islets, respectively, through the hydrophobic interaction between a lipid unit of the conjugates and the cell membrane. The Sertoli cells were immobilized on the islets through hybridization between oligo(dA)_(20) and oligo(dT)_(20). When Sertoli cell-immobilized islets were infused into the liver of mice through the portal vein, the Sertoli cells remained around the islets.
机译：睾丸支持细胞在创造睾丸的免疫特权环境中起着至关重要的作用。我们与支持细胞共移植后检查了Langerhans胰岛的存活。胰岛附近的支持细胞应保护移植物免受排斥。在这项研究中，单链寡核苷酸，聚（乙二醇）和磷脂（ssDNA-PEG-DPPE）的缀合物用于将Sertoli细胞固定在胰岛上。将20聚体脱氧腺苷酸（oligo（dA）_（20））和20聚体脱氧胸苷酸（oligo（dT）_（20））分别作为ssDNA呈递到Sertoli细胞和胰岛的表面。缀合物的脂质单元与细胞膜之间的疏水相互作用。 Sertoli细胞通过oligo（dA）_（20）和oligo（dT）_（20）之间的杂交固定在胰岛上。当通过门静脉将固定有Sertoli细胞的胰岛注入小鼠肝脏时，Sertoli细胞仍保留在胰岛周围。

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