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首页> 外文期刊>Platelets >Disaggregatory effects of prostaglandin E1, amrinone and milrinone on platelet aggregation in human whole blood.
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Disaggregatory effects of prostaglandin E1, amrinone and milrinone on platelet aggregation in human whole blood.

机译：前列腺素E1，氨力农和米力农对人全血中血小板聚集的分解作用。

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摘要

Prostaglandin E1 (PGE1), amrinone and milrinone not only inhibit platelet aggregation, but also potentially induce disaggregation. We examined the disaggregatory effects of PGE1, amrinone and milrinone on platelet aggregation, and measured the platelet cyclic adenosine monophosphate (cAMP) levels using a standard radioimmunoassay. Platelet aggregation was induced by 10 microM adenosine diphosphate (ADP) or 0.625 microg/ml collagen that induces a secondary aggregation, and measured by whole blood aggregometry. PGE1 (0.7-10.0 microM), amrinone (106.8-801.3 microM), or milrinone (9.5-190.0 microM) was added when aggregation reached 5 mohm of impedance, and the percentage of disaggregation was measured 5 min after initiating disaggregation. Disaggregatory effects were also examined using a light transmission aggregometer and gel-filtered platelets. In platelet aggregation induced by ADP, the drug concentrations required to cause 50% disaggregation (EC50) were PGE1; 3.1+/-1.2 microM, amrinone; 313.1+/-128.8 microM, milrinone; 57.1+/-20.8 microM. In platelet aggregation induced by collagen, the maximum disaggregation percentages were 15.6+/-5.0% for 10 microM PGE1, 7.8+/-2.4% for 801.3 microM amrinone, and 5.9+/-2.3% for 190.0 microM milrinone. The EC50 concentrations of platelet cAMP that caused 50% disaggregation in ADP-induced platelet aggregation were 67+/-13 pmoles/10(8) platelets for PGE1, 54+/-12 pmoles/10(8) platelets for amrinone, and 52+/-12 pmoles/10(8) platelets for milrinone. In gel-filtered platelets, the percentages of disaggregation in ADP- or collagen-induced platelet aggregation were 33.1+/-5.2% or 11.2+/-3.1% for PGE1 (10 microM), 22.3+/-4.1% or 15.2+/-3.2% for amrinone (801.3 microM), and 23.5+/-4.3% or 14.6+/-3.5% for milrinone (190.0 microM).
机译：前列腺素E1（PGE1），氨力农和米力农不仅抑制血小板凝集，而且还可能引起解聚。我们检查了PGE1，氨力农和米力农对血小板聚集的分解作用，并使用标准放射免疫测定法测量了血小板环一磷酸腺苷（cAMP）的水平。血小板聚集是由10 microM腺苷二磷酸（ADP）或0.625 microg / ml胶原蛋白诱导的继发性聚集诱导的，并通过全血凝集测定。当聚集达到5 mohm的阻抗时，添加PGE1（0.7-10.0 microM），氨力农（106.8-801.3 microM）或米力农（9.5-190.0 microM），并在开始聚集后5分钟测量分离百分比。还使用透光聚集仪和凝胶过滤的血小板检查了分解作用。在ADP诱导的血小板聚集中，引起50％分解（EC50）所需的药物浓度为PGE1; 3.1 +/- 1.2 microM，氨力农; 313.1 +/- 128.8 microM，米力农; 57.1 +/- 20.8 microM。在胶原蛋白诱导的血小板聚集中，最大分解百分率对于10 microM PGE1是15.6 +/- 5.0％，对于801.3 microM氨力农是7.8 +/- 2.4％，对于190.0 microM米力农是5.9 +/- 2.3％。在ADP诱导的血小板聚集中引起50％聚集的血小板cAMP的EC50浓度对于PGE1是67 +/- 13 pmoles / 10（8）血小板，对于氨力农是54 +/- 12 pmoles / 10（8）血小板，52米力农的+/- 12 pmoles / 10（8）血小板。在凝胶过滤的血小板中，PGE1（10 microM），ADP或胶原蛋白诱导的血小板聚集中的聚集百分比分别为33.1 +/- 5.2％或11.2 +/- 3.1％，22.3 +/- 4.1％或15.2 + /氨力农（801.3 microM）为-3.2％，米力农（190.0 microM）为23.5 +/- 4.3％或14.6 +/- 3.5％。

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