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Effect of centrifugation time on growth factor and MMP release of an experimental platelet-rich fibrin-type product

机译：离心时间对实验性富血小板纤维蛋白型产品生长因子和MMP释放的影响

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Platelet-rich fibrin (PRF) has a controlled release of growth factors due to the fibrin matrix structure. Different centrifugation protocols were suggested for PRF preparation. Since the derivation method of PRF can alter its contents, in the present study it is aimed to investigate the cell contents and transforming growth factor beta-1 (TGF-beta 1), platelet-derived growth factor (PDGF-AB), vascular endothelial growth factor (VEGF), matrix metalloproteinase (MMP)-1 and-8 release from experimental PRF-type membranes obtained with different centrifugation times at 400 gravity. Three blood samples were collected from 20 healthy non-smoker volunteers. One tube was used for whole blood analyses. The other two tubes were centrifuged at 400 g for 10 minutes (group A) or 12 minutes (group B). Each experimental PRF-type membrane was placed in Dulbecco's Modified Eagle's Medium (DMEM) and at 1, 24 and 72 hours, TGF-beta 1, PDGF-AB, VEGF, MMP-1 and -8 release amounts were analysed by enzyme-linked immunosorbent assay (ELISA). The blood cell count of membranes was determined by subtracting plasma supernatant and red blood cell (RBC) mixture from the whole blood cell counts. At 72 hours, the VEGF level of group B was statistically higher than that of group A (p = 0.040). The centrifugation time was not found to influence the release of other growth factors, enzymes and cell counts. Within the limits of the present study, it might be suggested that centrifugation time at a constant gravity has a significant effect on the VEGF levels released from experimental PRF-type membrane. It can be concluded that due to the importance of VEGF in the tissue healing process, membranes obtained at 12-minute centrifugation time may show a superior potential in wound healing.
机译：富含血小板的纤维蛋白（PRF）由于纤维蛋白基质结构而具有受控的生长因子释放。建议使用不同的离心方案制备PRF。由于PRF的衍生方法可以改变其含量，因此本研究旨在研究细胞含量和转化生长因子β-1（TGF-β1），血小板源性生长因子（PDGF-AB），血管内皮细胞生长因子（VEGF），基质金属蛋白酶（MMP）-1和-8从在400重力下不同离心时间获得的实验PRF型膜中释放。从20名健康的非吸烟志愿者那里采集了三份血液样本。一管用于全血分析。将另两个试管以400 g离心10分钟（A组）或12分钟（B组）。将每种实验的PRF型膜置于Dulbecco的改良Eagle's培养基（DMEM）中，并在1、24和72小时时，通过酶联法分析TGF-beta 1，PDGF-AB，VEGF，MMP-1和-8的释放量免疫吸附测定（ELISA）。通过从全血细胞计数中减去血浆上清液和红细胞（RBC）混合物来确定膜的血细胞计数。在72小时时，B组的VEGF水平在统计学上高于A组（p = 0.040）。未发现离心时间会影响其他生长因子，酶和细胞计数的释放。在本研究的范围内，可能表明恒定重力下的离心时间对从实验PRF型膜释放的VEGF水平有重大影响。可以得出结论，由于VEGF在组织愈合过程中的重要性，在12分钟的离心时间获得的膜可能在伤口愈合中显示出更高的潜力。

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