...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Platelets >Increased platelet purinergic sensitivity in peripheral arterial disease - A pilot study.
【24h】

Increased platelet purinergic sensitivity in peripheral arterial disease - A pilot study.

机译：外周动脉疾病中血小板嘌呤能敏性增加-一项初步研究。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Peripheral arterial disease (PAD) is associated with platelet hyperaggregability as well as an increase in morbidity and mortality from myocardial infarction (MI) and stroke. Purinergic signaling has been shown, both experimentally and clinically, to play an important role in the activation of platelets. Platelets express three different purinergic receptors: P2Y1, P2Y12 and P2X1. We assessed the hypothesis that the hyperaggregability associated with PAD is partly due to an increased P2 receptor expression at the transcriptional and/or translational level. Patients with PAD (n?=?8) and controls (n?=?8) were studied. Using a high-resolution channelyzer, platelet shape change (PSC) was assessed by measuring the median platelet volume (MPV). The fall in free platelet count following the addition of ADP was also assessed. Real-time PCR was used to quantify the mRNA expression and Western blots to quantify the protein expression of P2 receptors in platelets. The median (and range) fall in free platelet count after adding ADP was significantly (P?=?0.02) greater for patients [11% (5-24); n?=?8] than for controls [0.5% (0-10); n?=?8] despite using a lower concentration of ADP for the patient samples. The MPV did not differ significantly. The mRNA levels for the three P2 receptors were similar in PAD patients and controls. Western blot detected no significant differences in protein expression between these groups. Thus, platelets from PAD patients show an increased activation after stimulation with ADP (even though all patients were on aspirin). This hyperactivity was neither due to an obvious up-regulation of the mRNA levels nor to altered protein levels of P2 receptors in the platelets. It is suggested that the increased sensitivity to ADP in PAD is caused by post-receptor mechanisms.
机译：周围动脉疾病（PAD）与血小板高凝集性以及心肌梗塞（MI）和中风的发病率和死亡率增加相关。在实验和临床上都显示嘌呤能信号在血小板活化中起重要作用。血小板表达三种不同的嘌呤能受体：P2Y1，P2Y12和P2X1。我们评估了以下假设：与PAD相关的过度聚集性部分是由于在转录和/或翻译水平上P2受体表达增加所致。研究了PAD（n≥8）和对照组（n≥8）的患者。使用高分辨率通道分析仪，通过测量中位血小板体积（MPV）评估血小板形状变化（PSC）。还评估了添加ADP后游离血小板计数的下降。实时PCR被用于定量mRNA表达和蛋白质印迹以定量P2受体在血小板中的蛋白表达。加入ADP后患者的游离血小板计数下降的中位数（和范围）显着更大（P == 0.02）[11％（5-24）; n？=？8]，比对照[0.5％（0-10）;尽管对于患者样品使用较低浓度的ADP，但是n≥8。 MPV没有显着差异。在PAD患者和对照组中，三种P2受体的mRNA水平相似。 Western印迹检测到这些组之间的蛋白质表达没有显着差异。因此，PAD患者的血小板在ADP刺激后显示出增强的激活作用（即使所有患者都服用阿司匹林）。这种多动性既不是由于mRNA水平的明显上调也不是由于血小板中P2受体的蛋白质水平改变。提示PAD对ADP的敏感性增加是由于受体后机制引起的。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号