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首页> 外文期刊>Platelets >Leukocyte count and leukocyte ecto-nucleotidase are major determinants of the effects of adenosine triphosphate and adenosine diphosphate on platelet aggregation in human blood.
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Leukocyte count and leukocyte ecto-nucleotidase are major determinants of the effects of adenosine triphosphate and adenosine diphosphate on platelet aggregation in human blood.

机译：白细胞计数和白细胞外切核苷酸酶是三磷酸腺苷和二磷酸腺苷对人体血液中血小板聚集作用的主要决定因素。

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ADP induces platelet aggregation in human whole blood and platelet-rich plasma (PRP). ATP induces aggregation in whole blood only; this involves leukocytes and is mediated by ADP. Here we studied ATP- and ADP-induced aggregation in patients with raised leukocyte counts (mean 46.2x10(3) leukocytes/microl). Platelet aggregation was measured by platelet counting. ATP, ADP and metabolites were measured by HPLC. Aggregation to ADP (1-10 microM) and ATP (10-100 microM) was markedly reduced, but to ATP (1000 microM) was enhanced (all p<0.001). Aggregation to ADP in PRP was normal. Increasing the leukocyte count in normal blood reproduced the findings in the patients. Adding leukocytes (either MNLs or PMNLs) to normal PRP enabled a response to ATP and caused marked inhibition of ADP-induced aggregation. Breakdown of ATP or ADP to AMP and adenosine in leukocyte-rich plasma was rapid (t1/2=4 min) and far higher than in cell-free plasma or PRP. With ATP there was also formation of ADP, maximal at 4 min. The presence of the ectonucleotidase NTPDase1 (CD39) was demonstrated on MNLs (all of the monocytes and a proportion of the lymphocytes) and all PMNLs by flow cytometry. We conclude that leukocytes provide a means of dephosphorylating ATP which enables ATP-induced aggregation via conversion to ADP, but also convert ADP to AMP and adenosine. Platelet aggregation extent is a balance between these activities, and high white cell counts influence this balance.
机译：ADP诱导人全血和富血小板血浆（PRP）中的血小板聚集。 ATP仅诱导全血聚集;这涉及白细胞，并由ADP介导。在这里，我们研究了白细胞计数升高的患者（平均46.2x10（3）白细胞/微升）中ATP和ADP诱导的聚集。通过血小板计数测量血小板聚集。通过HPLC测量ATP，ADP和代谢产物。到ADP（1-10 microM）和ATP（10-100 microM）的聚集显着减少，但是到ATP（1000 microM）的聚集得到增强（所有p <0.001）。在PRP中聚集到ADP是正常的。正常人血液中白细胞计数的增加重现了患者的发现。将白细胞（MNL或PMNL）添加到正常的PRP中可实现对ATP的反应，并显着抑制ADP诱导的聚集。在富含白细胞的血浆中，ATP或ADP分解为AMP和腺苷的速度很快（t1 / 2 = 4分钟），远高于无细胞血浆或PRP。使用ATP，也形成了ADP，在4分钟时最大。流式细胞术在MNLs（所有单核细胞和一部分淋巴细胞）和所有PMNLs上证实了胞外核苷酸酶NTPDase1（CD39）的存在。我们得出的结论是白细胞提供了一种使ATP脱磷酸的方法，该方法使ATP可以通过转化为ADP来诱导聚集，而且还可以将ADP转化为AMP和腺苷。血小板聚集程度是这些活动之间的平衡，而高白细胞计数会影响这种平衡。

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