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首页> 外文期刊>Platelets >Flow cytometric assay of platelet glycoprotein receptor numbers in hypercholesterolemia.
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Flow cytometric assay of platelet glycoprotein receptor numbers in hypercholesterolemia.

机译：高胆固醇血症中血小板糖蛋白受体数量的流式细胞术分析。

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In this study, platelet glycoprotein (Gp) receptor numbers were measured by a flow cytometric assay using Cytoquant Gp in seven hypercholesterolemic and five normal subjects. Thrombin receptor agonist peptide (TRAP) was used to activate platelets. In hypercholesterolemia the Gp receptor numbers per resting platelet were found to be: 38 629 +/- 8538 (GpIIb/IIIa), 22 269 +/- 5628 (GpIb), 37 037 +/- 9810 (GpIIIa), 224 +/- 504 (CD62-P). After activation, receptor numbers were determined to be: 56 399 +/- 9003 (GpIIb/IIIa), 10 970 +/- 5319 (GpIb), 50 715 +/- 7904 (GpIIIa), 1222 +/- 687 (P-selectin). In the normal group before the activation, receptor numbers were: 43 828 +/- 8862 (GpIIb/IIIa), 22 166 +/- 3847 (GpIb), 42 351 +/- 1049 (GpIIIa), 62 +/- 139 (CD62-P), After activation, receptor numbers were determined to be: 60 573 +/- 4294 (GpIIb/IIIa), 13 003 +/- 4118 (GpIb), 52 067 +/- 1039 (GpIIIa), 3608 +/- 1508 (CD62-P). In hypercholesterolemic subjects, GpIIb/IIIa and GpIIIa receptor numbers on activated platelets increased significantly, whereas P-selectin numbers remained unchanged. However, the GpIb levels decreased significantly. In the control group, after activation, GpIIb/IIIa and P-selectin receptors increased significantly, GpIIIa receptor numbers did not change significantly, whereas GpIb receptor numbers decreased significantly. When the GpIIb/IIIa, GpIb, GpIIIa receptor numbers of the control group and hypercholesterolemic group were compared before and after activation, no significant changes were observed (P > 0.05). But P-selectin receptor numbers were significantly decreased in hypercholesterolemic patients compared to normals following TRAP activation (P < 0.05). In this study, the effect of hypercholesterolemia on platelet function was observed. The striking observation about present study was the marked decrease in P-selectin expression after activation in the hypercholesterolemics compared to normals. This finding suggests some sort of platelet dysfunction in these individuals.
机译：在这项研究中，通过流式细胞术测定了使用7种高胆固醇血症和5名正常受试者的Cytoquant Gp的血小板糖蛋白（Gp）受体的数量。凝血酶受体激动剂肽（TRAP）用于激活血小板。在高胆固醇血症中，每个静息血小板的Gp受体数为：38629 +/- 8538（GpIIb / IIIa），22 269 +/- 5628（GpIb），37037 +/- 9810（GpIIIa），224 +/- 504（CD62-P）。激活后，受体数确定为：56399 +/- 9003（GpIIb / IIIa），10 970 +/- 5319（GpIb），50715 +/- 7904（GpIIIa），1222 +/- 687（P-选择素）。在激活之前的正常组中，受体数量为：43 828 +/- 8862（GpIIb / IIIa），22 166 +/- 3847（GpIb），42 351 +/- 1049（GpIIIa），62 +/- 139（ CD62-P），激活后确定受体数为：60 573 +/- 4294（GpIIb / IIIa），1300 +/- 4118（GpIb），52067 +/- 1039（GpIIIa），3608 + / -1508（CD62-P）。在高胆固醇血症受试者中，活化血小板上的GpIIb / IIIa和GpIIIa受体数量显着增加，而P-选择素数量保持不变。但是，GpIb水平显着下降。在对照组中，激活后，GpIIb / IIIa和P-选择素受体显着增加，GpIIIa受体数量没有显着变化，而GpIb受体数量显着下降。比较活化前后对照组和高胆固醇血症组的GpIIb / IIIa，GpIb，GpIIIa受体数量，未见明显变化（P> 0.05）。但是，与激活TRAP后的正常人相比，高胆固醇血症患者的P-选择素受体数量显着降低（P <0.05）。在这项研究中，观察到高胆固醇血症对血小板功能的影响。关于本研究的惊人发现是与正常人相比，高胆固醇血症患者激活后P-选择素表达显着下降。这一发现表明这些个体中存在某种血小板功能障碍。

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