• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Protein Science: A Publication of the Protein Society >Structural and functional features of an NDP kinase from the hyperthermophile crenarchaeon Pyrobaculum aerophilum.
【24h】

Structural and functional features of an NDP kinase from the hyperthermophile crenarchaeon Pyrobaculum aerophilum.

机译：来自嗜热嗜热菌嗜热热单生菌的NDP激酶的结构和功能特征。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Nucleoside diphosphate (NDP) kinases are ubiquitous enzymes that transfer gamma-phosphates from nucleoside triphosphates to nucleoside diphosphates via a ping-pong mechanism. The important role of this large family of enzymes in controlling cellular functions and developmental processes along with their crystallizability has made them good candidates for structural studies. We recently determined the structure of an evolved version of an NDP kinase from Pyrobaculum aerophilum, an extreme thermophile. This NDP kinase has similarity to the 42 other NDP kinases deposited in the Protein Data Bank (PDB) but differs significantly in sequence, structure, and biophysical properties. The P. aerophilum NDP kinase sequence contains two unique segments not present in other NDP kinases, comprising residues 66-100 and 156-165. We show that deletion mutants of the P. aerophilum NDP kinase lacking either or both of these inserts have an altered substrate specificity, allowing dGTP as the phosphate donor. A structural analysis of the evolved NDP kinase in conjunction with mutagenesis experiments suggests that the substrate specificity of the P. aerophilum NDP kinase is related to the presence of these two inserts.
机译：核苷二磷酸（NDP）激酶是一种普遍存在的酶，它通过乒乓机制将γ-磷酸酯从三磷酸核苷转移到二磷酸核苷。这个庞大的酶家族在控制细胞功能和发育过程中的重要作用及其可结晶性使其成为结构研究的良好候选者。最近，我们确定了嗜热嗜热菌（Pyrobaculum aerophilum）（一种极端嗜热菌）的NDP激酶进化版本的结构。此NDP激酶与存放在蛋白质数据库（PDB）中的其他42种NDP激酶相似，但在序列，结构和生物物理特性上有显着差异。嗜氧假单胞菌NDP激酶序列含有两个其他NDP激酶中不存在的独特区段，其包含残基66-100和156-165。我们显示缺少这些插入物中的一个或两个的P. aerophilum NDP激酶的缺失突变体具有改变的底物特异性，允许dGTP作为磷酸盐供体。结合诱变实验对进化的NDP激酶的结构分析表明，嗜氧假单胞菌NDP激酶的底物特异性与这两个插入片段的存在有关。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号