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首页> 外文期刊>Biomaterials >BIOMATERIAL-INDUCED ALTERATIONS OF HUMAN NEUTROPHILS UNDER FLUID SHEAR STRESS - SCANNING ELECTRON MICROSCOPICAL STUDY IN VITRO
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BIOMATERIAL-INDUCED ALTERATIONS OF HUMAN NEUTROPHILS UNDER FLUID SHEAR STRESS - SCANNING ELECTRON MICROSCOPICAL STUDY IN VITRO

机译：流体剪切应力作用下人类中性粒细胞的生物材料诱导变化-扫描电镜观察

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Morphological changes of human polymorphonuclear neutrophils (PMN) adhering to hydrophilic (glass) and hydrophobic (FEP-Teflon, polyethylene, polypropylene) surfaces were studied in a parallel-plate flow chamber at the light and scanning electron microscopical levels. The PMN were exposed to a shear stress of 0.19 Pa (1.9 dynes cm(-2)) or were allowed to adhere without the stress component (static control) during 30 min for all four biomaterials. Observation by light microscopy was performed in situ in the flow chamber at 1, 5, 10, 15, 20, 25 and 30 min. The total number of adherent cells as a function of time and the activation status of the population on the basis of morphological criteria were determined. On the hydrophilic material adhesion of activated PMN was significantly higher (P < 0.05) than on the more hydrophobic surfaces. This effect was most pronounced for the adhesion of neutrophils to glass and polypropylene (PP). Polyethylene (PE) showed only minor adhesion rates. Scanning electron microscopy revealed details of cell shape changes and permitted a more precise classification of populations of neutrophils based on distinctive shapes. As PMN were exposed to shear stress on glass, the majority of cells exhibited surface veils, ridges and ruffles, suggesting a high level of cell migration. In this case, on polymeric surfaces the presence of filopodial networks (FEP-Teflon) and ameoboid cell shapes (PP and PE) was noted. The results suggest that a low shear stress, as well as various chemical and physical properties of biomaterial surfaces, are together responsible for differentiation of PMN populations on solid substrata. (C) 1996 Elsevier Science Limited [References: 36]
机译：在光和扫描电子显微镜水平下，研究了在平行板流动室中粘附于亲水（玻璃）和疏水（FEP-Teflon，聚乙烯，聚丙烯）表面的人类多形核中性粒细胞（PMN）的形态变化。对于所有四种生物材料，PMN暴露于0.19 Pa（1.9达因厘米（-2））的剪切应力下，或者在30分钟内不施加应力分量的情况下粘附（静态控制）。在1、5、10、15、20、25和30分钟时，在流动室中进行光学显微镜观察。根据形态学标准，确定随时间变化的贴壁细胞总数和群体的活化状态。在亲水性材料上，活化的PMN的粘附性比在疏水性更强的表面上显着更高（P <0.05）。这种作用在嗜中性粒细胞对玻璃和聚丙烯（PP）的粘附中最为明显。聚乙烯（PE）仅显示较小的粘合率。扫描电子显微镜揭示了细胞形状变化的细节，并允许根据独特的形状对嗜中性粒细胞群体进行更精确的分类。当PMN暴露于玻璃上的剪切应力时，大多数细胞表现出表面面纱，脊和褶皱，表明细胞迁移水平很高。在这种情况下，注意到在聚合物表面上存在丝虫网络（FEP-Teflon）和变形虫细胞形状（PP和PE）。结果表明，低剪切应力以及生物材料表面的各种化学和物理特性共同导致了固体基质上PMN种群的分化。 （C）1996 Elsevier Science Limited [参考：36]

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