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首页> 外文期刊>Proteomics >Distinct serum proteome profiles associated with collagen-induced arthritis and complete Freund's adjuvant-induced inflammation in CD38(-/-) mice: The discriminative power of protein species or proteoforms
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Distinct serum proteome profiles associated with collagen-induced arthritis and complete Freund's adjuvant-induced inflammation in CD38(-/-) mice: The discriminative power of protein species or proteoforms

机译：与胶原蛋白诱导的关节炎和完全弗氏佐剂诱导的CD38（-/-）小鼠炎症相关的独特血清蛋白组谱：蛋白质种类或蛋白形式的判别力

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摘要

Collagen-type-II-induced arthritis (CIA) is an autoimmune disease, which involves a complex host systemic response including inflammatory and autoimmune reactions. CIA is milder in CD38(-/-) than in wild-type (WT) mice. ProteoMiner-equalized serum samples were subjected to 2D-DiGE and MS-MALDI-TOF/TOF analyses to identify proteins that changed in their relative abundances in CD38(-/-) versus WT mice either with arthritis (CIA(+)), with no arthritis (CIA(-)), or with inflammation (complete Freund's adjuvant (CFA)-treated mice). Multivariate analyses revealed that a multiprotein signature (n = 28) was able to discriminate CIA(+) from CIA(-) mice, and WT from CD38(-/-) mice within each condition. Likewise, a distinct multiprotein signature (n = 16) was identified which differentiated CIA(+)CD38(-/-) mice from CIA(+) WT mice, and lastly, a third multiprotein signature (n = 18) indicated that CD38(-/-) and WT mice could be segregated in response to CFA treatment. Further analyses showed that the discriminative power to distinguish these groups was reached at protein species level and not at the protein level. Hence, the need to identify and quantify proteins at protein species level to better correlate proteome changes with disease processes. It is crucial for plasma proteomics at the low-abundance protein species level to apply the ProteoMiner enrichment. All MS data have been deposited in the ProteomeXchange with identifiers PXD001788, PXD001799 and PXD002071 (, and ).
机译：II型胶原蛋白诱发的关节炎（CIA）是一种自身免疫性疾病，涉及复杂的宿主系统反应，包括炎症和自身免疫反应。 CIA在CD38（-/-）中比在野生型（WT）小鼠中轻。对ProteoMiner平衡的血清样品进行2D-DiGE和MS-MALDI-TOF / TOF分析，以鉴定CD38（-/-）相对于患有关节炎（CIA（+））的WT小鼠的相对丰度发生变化的蛋白质。无关节炎（CIA（-））或有炎症（完全弗氏佐剂（CFA）处理的小鼠）。多变量分析显示，在每种情况下，多蛋白签名（n = 28）能够区分CIA（-）小鼠的CIA（+）和CD38（-/-）小鼠的WT。同样，鉴定出了独特的多蛋白特征（n = 16），该特征将CIA（+）CD38（-/-）小鼠与CIA（+）WT小鼠区分开，最后，第三个多蛋白特征（n = 18）表明CD38（ -/-）和WT小鼠可以响应CFA治疗而隔离。进一步的分析表明，在蛋白质种类级别而非蛋白质级别达到了区分这些组的区分能力。因此，需要在蛋白质种类水平上鉴定和定量蛋白质，以更好地将蛋白质组变化与疾病过程相关联。在蛋白质丰度低的血浆蛋白质组学中，应用ProteoMiner富集至关重要。所有MS数据均已存储在ProteomeXchange中，其标识符为PXD001788，PXD001799和PXD002071（和）。

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