• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Proteomics >Proteome analysis of multidrug resistance in vincristine-resistant human gastric cancer cell line SGC7901/VCR.
【24h】

Proteome analysis of multidrug resistance in vincristine-resistant human gastric cancer cell line SGC7901/VCR.

机译：耐长春新碱的人胃癌细胞系SGC7901 / VCR中多药耐药性的蛋白质组分析。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

In order to elucidate the mechanisms of multidrug resistance (MDR) of vincristine-resistant human gastric carcinoma cell line SGC7901/VCR, 2-DE was used to separate the total proteins of SGC7901/VCR and its parental cell line SGC7901. PDQuest software was applied to analyze 2-DE images, and the differential protein spots were identified by both MALDI-TOF-MS and ESI-Q-TOF-MS. Then the differential expressional levels of partially identified proteins were determined by Western blot analysis and real-time RT-PCR. Furthermore, the association of heat shock protein (HSP27), one of the highly expressed proteins in sgc7901/vcr, with MDR was analyzed using antisense inhibition of HSP27. In this study, the well-resolved, reproducible 2-DE patterns of SGC7901/VCR and SGC7901 were established, and yielded about 1100 protein-spots each. All the 24 differential proteins between the two cell lines were identified, and the differential expression levels of the partial proteins were confirmed. The suppression of HSP27expression by HSP27 antisense oligonucleotides could enhance vincristine chemosensitivity in sgc7901/vcr and induce the cells to exhibit apoptotic morphological features after vincristine treatment. The differentially expressed proteins could be divided into six groups based on their functions: calcium-binding proteins, chaperones, proteins involved in drug detoxification or repair of DNA damage, metabolic enzymes, proteins related to cellular structure, and proteins relative to signal transduction, some of which may contribute to MDR of human gastric carcinoma cell line SGC7901/VCR. These data will be valuable for further study of the mechanisms of MDR in human gastric cancer.
机译：为了阐明耐长春新碱的人胃癌细胞系SGC7901 / VCR的多药耐药性（MDR）机制，使用2-DE分离SGC7901 / VCR及其亲代细胞系SGC7901的总蛋白。应用PDQuest软件分析2-DE图像，并通过MALDI-TOF-MS和ESI-Q-TOF-MS识别差异蛋白斑点。然后通过蛋白质印迹分析和实时RT-PCR确定部分鉴定的蛋白质的差异表达水平。此外，使用HSP27的反义抑制作用分析了sgc7901 / vcr中高表达蛋白之一的热激蛋白（HSP27）与MDR的关联。在这项研究中，建立了SGC7901 / VCR和SGC7901的分辨良好，可重现的2-DE模式，每个模式产生约1100个蛋白斑点。鉴定了两个细胞系之间的所有24种差异蛋白，并确认了部分蛋白的差异表达水平。 HSP27反义寡核苷酸抑制HSP27表达可增强sgc7901 / vcr中长春新碱的化学敏感性，并诱导长春新碱处理后细胞具有凋亡的形态学特征。根据它们的功能，差异表达的蛋白质可以分为六类：钙结合蛋白，分子伴侣，参与药物排毒或修复DNA损伤的蛋白质，代谢酶，与细胞结构有关的蛋白质以及与信号转导有关的蛋白质，一些其中可能对人胃癌细胞SGC7901 / VCR的MDR有贡献。这些数据对于进一步研究人类胃癌中MDR的机制是有价值的。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号